TAF3/YPL011C Summary

Standard Name	TAF3 1
Systematic Name	YPL011C
Alias	TAF47
Feature Type	ORF, Verified
Description	TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation (1, 2 and see Summary Paragraph)
Name Description	TATA binding protein-Associated Factor
Gene Product Alias	TafII47

Chromosomal Location	ChrXVI:534703 to 533642 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All TAF3 GO evidence and references

	View Computational GO annotations for TAF3
Molecular Function
Manually curated	chromatin binding (IDA) contributes_to RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly (IC)
Biological Process
Manually curated	RNA polymerase II transcriptional preinitiation complex assembly (IC) transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	transcription factor TFIID complex (IDA)

Mutant phenotypes All TAF3 Phenotype evidence and references

Classical genetics
overexpression	viability: decreased
Large-scale survey
null	haploproficient inviable resistance to elesclomol-Cu: decreased
overexpression	filamentous growth: increased silencing: abnormal
reduction of function	competitive fitness: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All TAF3 Interaction evidence and references

	115 total interaction(s) for 28 unique genes/features.
Physical Interactions	Affinity Capture-MS: 80 Affinity Capture-RNA: 2 Affinity Capture-Western: 15 Co-localization: 1 Co-purification: 3 Two-hybrid: 4
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 6 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All TAF3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:534703 to 533642 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1062	534703..533642	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005932

SUMMARY PARAGRAPH for TAF3

About TFIID

TFIID is a transcription factor complex that is required for RNAPII-mediated transcription of protein-coding genes and some small nuclear RNAs (reviewed in 3). The complex is composed of Spt15p (TATA binding protein; TBP) and 14 TBP-associated factors (TAFs): Taf1p, Taf2p, Taf3p, Taf4p, Taf5p, Taf6p, Taf7p, Taf8p, Taf9p, Taf10p, Taf11p, Taf12p, Taf13p, Taf14p (4, 5). The TFIID complex is required for basal transcription, but some individual subunits regulate the activated transcription of a subset of genes (6, 7, 8, 9, 10).

Recognition of promoter DNA by the TFIID complex is required for the formation of the preinitiation complex (PIC) during transcription initiation (11, 12). The interaction between the TFIID complex and the promoter is stabilized by TFIIA (12, 13). The recruitment of TFIID to promoters is dependent on an upstream activating sequence in the promoter region (14).

A subset of the TAFs (Taf5p, Taf6p, Taf9p, Taf10p, and Taf12p) are subunits of both TFIID and the the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional regulatory complex, which functions in nucleosomal histone acetylation and chromatin-associated transcriptional activation or repression (15, 16, 17). The results of genome-wide studies indicate that TFIID functions primarily at the TATA-less promoters of stress-repressed housekeeping genes, representing about 90% of the yeast genome, while SAGA predominates at highly-regulated, stress-responsive TATA box-containing genes, representing about 10% of the genome (18, 19).

Last updated: 2009-10-02

References cited on this page View Complete Literature Guide for TAF3

1)	Tora L (2002) A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev 16(6):673-5
2)	Lee TI and Young RA (2000) Transcription of eukaryotic protein-coding genes. Annu Rev Genet 34:77-137
3)	Tansey WP and Herr W (1997) TAFs: guilt by association? Cell 88(6):729-32
4)	Sanders SL, et al. (2002) Molecular characterization of Saccharomyces cerevisiae TFIID. Mol Cell Biol 22(16):6000-13
5)	Auty R, et al. (2004) Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81
6)	Sayre MH, et al. (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82
7)	Macpherson N, et al. (2000) A yeast taf17 mutant requires the Swi6 transcriptional activator for viability and shows defects in cell cycle-regulated transcription. Genetics 154(4):1561-76
8)	Kobayashi A, et al. (2003) Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP). Nucleic Acids Res 31(4):1261-74
9)	Klebanow ER, et al. (1996) Isolation and characterization of TAF25, an essential yeast gene that encodes an RNA polymerase II-specific TATA-binding protein-associated factor. J Biol Chem 271(23):13706-15
10)	Walker SS, et al. (1997) Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state. Cell 90(4):607-14
11)	Shen WC, et al. (2003) Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly. EMBO J 22(13):3395-402
12)	Buratowski S, et al. (1989) Five intermediate complexes in transcription initiation by RNA polymerase II. Cell 56(4):549-61
13)	Ranish JA and Hahn S (1991) The yeast general transcription factor TFIIA is composed of two polypeptide subunits. J Biol Chem 266(29):19320-7
14)	Li XY, et al. (2002) Selective recruitment of TAFs by yeast upstream activating sequences. Implications for eukaryotic promoter structure. Curr Biol 12(14):1240-4
15)	Grant PA, et al. (1998) A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell 94(1):45-53
16)	Pray-Grant MG, et al. (2002) The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol 22(24):8774-86
17)	Warfield L, et al. (2004) Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA. Genes Dev 18(9):1022-34
18)	Huisinga KL and Pugh BF (2004) A genome-wide housekeeping role for TFIID and a highly regulated stress-related role for SAGA in Saccharomyces cerevisiae. Mol Cell 13(4):573-85
19)	Basehoar AD, et al. (2004) Identification and distinct regulation of yeast TATA box-containing genes. Cell 116(5):699-709