LSP1/YPL004C Summary Help

Standard Name LSP1 1, 2
Systematic Name YPL004C
Feature Type ORF, Verified
Description Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family (2, 3, 4, 5)
Name Description Long chain bases Stimulate Phosphorylation 2
Chromosomal Location
ChrXVI:551657 to 550632 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All LSP1 GO evidence and references
  View Computational GO annotations for LSP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
153 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 79
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-localization: 4
  • PCA: 23
  • Reconstituted Complex: 2
  • Two-hybrid: 13

Genetic Interactions
  • Negative Genetic: 15
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 341
Molecular Weight (Da) 38,071
Isoelectric Point (pI) 4.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:551657 to 550632 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1026 551657..550632 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005925
References cited on this page View Complete Literature Guide for LSP1
1) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2) Zhang X, et al.  (2004) Pil1p and Lsp1p negatively regulate the 3-phosphoinositide-dependent protein kinase-like kinase Pkh1p and downstream signaling pathways Pkc1p and Ypk1p. J Biol Chem 279(21):22030-8
3) Walther TC, et al.  (2006) Eisosomes mark static sites of endocytosis. Nature 439(7079):998-1003
4) Ziolkowska NE, et al.  (2011) Eisosome-driven plasma membrane organization is mediated by BAR domains.LID - 10.1038/nsmb.2080 [doi] Nat Struct Mol Biol ()
5) Mascaraque V, et al.  (2013) Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components. Mol Cell Proteomics 12(3):557-74