GDH1/YOR375C Summary Help

GDH1 BASIC INFORMATION

Standard Name GDH1
Systematic Name YOR375C
Alias URE1 , DHE4
Feature Type ORF, Verified
Description NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources (1, 2 and see Summary Paragraph)
Also known as: GDH-A , GDHA
Name Description Glutamate DeHydrogenase
GO Annotations All GDH1 GO evidence and references
    View Computational GO annotations for GDH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant Phenotype All GDH1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions GDH1 All interactions details and references
24 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Co-purification: 1
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1
Sequence Information
ChrXV:1043040 to 1041676 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1365 1043040..1041676 2006-01-05 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005902

GDH1 RESOURCES

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SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for GDH1

SUMMARY PARAGRAPH for GDH1

About glutamate biosynthesis from ammonia

Yeast cells contain 3 pathways for the synthesis of glutamate. Two pathways are mediated by two isoforms of glutamate dehydrogenase, encoded by GDH1 and GDH3 (3, 4). The third pathway is driven by the combined activities of glutamine synthetase and glutamate synthase, encoded by GLN1 and GLT1, respectively (5, 6). Studies of GDH1 and GDH3 regulation indicate that the cell uses these isoforms under different growth conditions (1). Expression of GDH3 is induced by ethanol and repressed by glucose, whereas GDH1 expression is high in either carbon source. Gdh1p uses alpha-ketoglutarate at a higher rate than Gdh3p. Thus, under fermentative growth conditions, Gdh1p drives glutamate biosynthesis, whereas in nonfermentable or limiting carbon sources, Gdh3p is the key isoform involved in balancing distribution of alpha-ketoglutarate to glutamate biosynthesis and energy metabolism.

Last updated: 2007-10-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GDH1]

1) DeLuna A, et al.  (2001) NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. J Biol Chem 276(47):43775-83
2) Riego L, et al.  (2002) GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Biochem Biophys Res Commun 293(1):79-85
3) Moye WS, et al.  (1985) Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. J Biol Chem 260(14):8502-8
4) Avendano A, et al.  (1997) GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. J Bacteriol 179(17):5594-7
5) Benjamin PM, et al.  (1989) Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet 217(2-3):370-7
6) Filetici P, et al.  (1996) Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase. Yeast 12(13):1359-66