GDH1/YOR375C Summary

Standard Name	GDH1
Systematic Name	YOR375C
Alias	URE1 , DHE4
Feature Type	ORF, Verified
Description	NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph) Also known as: GDH-A , GDHA
Name Description	Glutamate DeHydrogenase

Chromosomal Location	ChrXV:1043042 to 1041678 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GDH1 GO evidence and references

	View Computational GO annotations for GDH1
Molecular Function
Manually curated	glutamate dehydrogenase (NADP+) activity (IDA, IMP)
Biological Process
Manually curated	ammonia assimilation cycle (IEP) glutamate biosynthetic process (IGI)
Cellular Component
Manually curated	nucleus (IDA)
High-throughput	cytoplasm (IDA)

Pathways	glutamate biosynthesis from ammonia superpathway of glutamate biosynthesis

Mutant phenotypes All GDH1 Phenotype evidence and references

Classical genetics
null	alpha-ketoglutarate accumulation: increased growth in exponential phase: normal rate Gap1p distribution: abnormal redox state: abnormal vegetative growth: decreased rate
Large-scale survey
null	glutathione excretion: increased competitive fitness: decreased haploinsufficient Rad52-YFP distribution: increased resistance to ethanol: decreased respiratory growth: absent starvation resistance: decreased stress resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GDH1 Interaction evidence and references

	59 total interaction(s) for 47 unique genes/features.
Physical Interactions	Affinity Capture-MS: 23 Affinity Capture-RNA: 2 Co-purification: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 9 Phenotypic Enhancement: 3 Phenotypic Suppression: 1 Positive Genetic: 11 Synthetic Growth Defect: 4 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GDH1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:1043042 to 1041678 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1365	1043042..1041678	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005902

SUMMARY PARAGRAPH for GDH1

About glutamate biosynthesis from ammonia

Yeast cells contain 3 pathways for the synthesis of glutamate. Two pathways are mediated by two isoforms of glutamate dehydrogenase, encoded by GDH1 and GDH3 (4, 5). The third pathway is driven by the combined activities of glutamine synthetase and glutamate synthase, encoded by GLN1 and GLT1, respectively (6, 7). Studies of GDH1 and GDH3 regulation indicate that the cell uses these isoforms under different growth conditions (1). Expression of GDH3 is induced by ethanol and repressed by glucose, whereas GDH1 expression is high in either carbon source. Gdh1p uses alpha-ketoglutarate at a higher rate than Gdh3p. Thus, under fermentative growth conditions, Gdh1p drives glutamate biosynthesis, whereas in nonfermentable or limiting carbon sources, Gdh3p is the key isoform involved in balancing distribution of alpha-ketoglutarate to glutamate biosynthesis and energy metabolism.

Last updated: 2007-10-04

References cited on this page View Complete Literature Guide for GDH1

1)	DeLuna A, et al. (2001) NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles. J Biol Chem 276(47):43775-83
2)	Riego L, et al. (2002) GDH1 expression is regulated by GLN3, GCN4, and HAP4 under respiratory growth. Biochem Biophys Res Commun 293(1):79-85
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Moye WS, et al. (1985) Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. J Biol Chem 260(14):8502-8
5)	Avendano A, et al. (1997) GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. J Bacteriol 179(17):5594-7
6)	Benjamin PM, et al. (1989) Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet 217(2-3):370-7
7)	Filetici P, et al. (1996) Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase. Yeast 12(13):1359-66