GPB1/YOR371C Summary

GPB1/YOR371C Summary

Standard Name	GPB1 1
Systematic Name	YOR371C
Alias	KRH2 2
Feature Type	ORF, Verified
Description	Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)

Chromosomal Location	ChrXV:1034182 to 1031489 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GPB1 GO evidence and references

	View Computational GO annotations for GPB1
Molecular Function
Manually curated	cAMP-dependent protein kinase inhibitor activity (IGI) GTPase activating protein binding (IPI) signal transducer activity (IPI)
Biological Process
Manually curated	glucose mediated signaling pathway (IGI, IMP) invasive growth in response to glucose limitation (IGI, IMP) negative regulation of phosphorylation (IGI) negative regulation of Ras protein signal transduction (IGI) positive regulation of Ras protein signal transduction (IMP) protein polyubiquitination (IDA) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IDA, IMP) pseudohyphal growth (IGI, IMP) signal transduction (IPI)
Cellular Component
Manually curated	plasma membrane (IGI)
High-throughput	cytoplasm (IDA)

Mutant phenotypes All GPB1 Phenotype evidence and references

Classical genetics
null	invasive growth: increased Ira2p accumulation: increased pseudohyphal growth: increased
overexpression	Ras2p-GTP accumulation: increased Ira2p accumulation: decreased Ira2p modification: increased pseudohyphal growth: decreased
Large-scale survey
null	auxotrophy competitive fitness: increased dessication resistance: decreased haploproficient resistance to clioquinol: decreased resistance to arsenite(3-): decreased resistance to sodium arsenite: decreased resistance to ciclopirox olamine: decreased stress resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GPB1 Interaction evidence and references

	24 total interaction(s) for 19 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-Western: 5 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 7 Phenotypic Enhancement: 2 Phenotypic Suppression: 3 Positive Genetic: 1 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GPB1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:1034182 to 1031489 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2694	1034182..1031489	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005898

References cited on this page View Complete Literature Guide for GPB1

1)	Harashima T and Heitman J (2002) The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell 10(1):163-73
2)	Batlle M, et al. (2003) Krh1p and Krh2p act downstream of the Gpa2p G(alpha) subunit to negatively regulate haploid invasive growth. J Cell Sci 116(Pt 4):701-10
3)	Lu A and Hirsch JP (2005) Cyclic AMP-independent regulation of protein kinase A substrate phosphorylation by Kelch repeat proteins. Eukaryot Cell 4(11):1794-800
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Harashima T, et al. (2006) The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2. Mol Cell 22(6):819-30
6)	Peeters T, et al. (2006) Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast. Proc Natl Acad Sci U S A 103(35):13034-9
7)	Phan VT, et al. (2010) The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol 30(9):2264-79