PRT1/YOR361C Summary Help

Standard Name PRT1
Systematic Name YOR361C
Alias CDC63 1 , DNA26 2
Feature Type ORF, Verified
Description eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes (3, 4, 5, 6)
Name Description PRoTein synthesis
Chromosomal Location
ChrXV:1017652 to 1015361 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 221 cM
Gene Ontology Annotations All PRT1 GO evidence and references
  View Computational GO annotations for PRT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
reduction of function
285 total interaction(s) for 115 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 131
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 68
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 7
  • Co-purification: 9
  • PCA: 1
  • Reconstituted Complex: 19
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 18
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 4

Expression Summary
Length (a.a.) 763
Molecular Weight (Da) 88,129
Isoelectric Point (pI) 5.88
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:1017652 to 1015361 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 221 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2292 1017652..1015361 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005888
References cited on this page View Complete Literature Guide for PRT1
1) Bedard DP, et al.  (1981) New mutations in the yeast Saccharomyces cerevisiae affecting completion of "start". Curr Genet 4(3):205-14
2) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
3) Phan L, et al.  (1998) Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5. Mol Cell Biol 18(8):4935-46
4) Naranda T, et al.  (1994) Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein. J Biol Chem 269(51):32286-92
5) Keierleber C, et al.  (1986) Isolation and characterization of PRT1, a gene required for the initiation of protein biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol 6(12):4419-24
6) Phan L, et al.  (2001) A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J 20(11):2954-65