VMA4/YOR332W Summary Help

Standard Name VMA4
Systematic Name YOR332W
Feature Type ORF, Verified
Description Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress (1, 2, 3 and see Summary Paragraph)
Chromosomal Location
ChrXV:943656 to 944357 | ORF Map | GBrowse
Gene Ontology Annotations All VMA4 GO evidence and references
  View Computational GO annotations for VMA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
Large-scale survey
129 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 25
  • Biochemical Activity: 1
  • Co-crystal Structure: 3
  • Co-fractionation: 3
  • Co-purification: 3
  • Far Western: 2
  • PCA: 2
  • Protein-peptide: 7
  • Reconstituted Complex: 4
  • Two-hybrid: 7

Genetic Interactions
  • Phenotypic Enhancement: 4
  • Synthetic Growth Defect: 14
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 233
Molecular Weight (Da) 26,471
Isoelectric Point (pI) 5.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:943656 to 944357 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..702 943656..944357 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005859

VMA4 encodes the E subunit of the yeast V-ATPase V1 domain (1, 2). Vacuolar (H )-ATPases (V-ATPases) are ATP-dependent proton pumps that have been identified in many eukaryotes, where they acidify intracellular vacuolar compartments. Vacuolar acidification is important for many cellular processes, including endocytosis, targeting of newly synthesized lysosomal enzymes, and other molecular targeting processes. The V-ATPase consists of two separable domains. The V1 domain has eight known subunits, is peripherally associated with the vacuolar membrane, and catalyzes ATP hydrolysis. The V0 domain is an integral membrane structure of five subunits, and transports protons across the membrane. The structure, function, and assembly of V-ATPases are reviewed in references 4, 5, 6 and 7.

The vma4 null mutant is viable but lacks vacuolar (H )-ATPase activity and cannot grow at neutral pH or on nonfermentable carbon sources (1, 2). A temperature sensitive vma4 mutant, which loses V-ATPase activity and vacuolar acidification upon incubation at high temperatures, shows abnormal bud morphology and delocalization of actin and chitin at the restrictive temperature (8). Vma4p is required for the V1 domain to assemble onto the vacuolar membrane (2).CC,PH, PI,

Last updated: 2000-05-16 Contact SGD

References cited on this page View Complete Literature Guide for VMA4
1) Foury F  (1990) The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae. J Biol Chem 265(30):18554-60
2) Ho MN, et al.  (1993) Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase. J Biol Chem 268(1):221-7
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
5) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47
6) Kane PM  (1999) Biosynthesis and regulation of the yeast vacuolar H+-ATPase. J Bioenerg Biomembr 31(1):49-56
7) Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808
8) Zhang JW, et al.  (1998) Characterization of a temperature-sensitive yeast vacuolar ATPase mutant with defects in actin distribution and bud morphology. J Biol Chem 273(29):18470-80