SCD5/YOR329C Summary Help

Standard Name SCD5 1
Systematic Name YOR329C
Alias FTB1 2 , SCD7 1
Feature Type ORF, Verified
Description Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency (1, 3, 4 and see Summary Paragraph)
Name Description Suppressor of Clathrin Deficiency 5
Chromosomal Location
ChrXV:939349 to 936731 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SCD5 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
reduction of function
Large-scale survey
reduction of function
79 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 15
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 872
Molecular Weight (Da) 97,305
Isoelectric Point (pI) 7.94
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:939349 to 936731 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2619 939349..936731 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005856

Scd5p is an essential protein that colocalizes with cortical actin, and is required for cortical actin organization and endocytosis (6, 7, 3). Scd5p and the clathrin heavy- and light-chains (Chc1p & Clc1p) physically associate with Sla2p, a transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization, which is also a homolog of the mammalian huntingtin interacting protein HIP1 and the related HIP1R (6). Both Scd5p and clathrin are required for Sla2p localization at the cell cortex (6).

Scd5p activity appears to be phospho-regulated. Scd5p is phosphorylated by Prk1p, which results in its negative regulation, and dephosphorylated by the Glc7p type-1 protein phosphatase, which relieves this inhibition (7, 3, 8). Mutations in GLC7 that disrupt Glc7p interactions with Scd5p result in defects in endocytosis and actin organization (7, 3, 8).

Scd5p was first identified from a genetic screen searching for multicopy suppressors of defects seen in cells depleted of Chc1p (5). Loss of function scd5 mutants display defects in receptor-mediated endocytosis, fluid-phase endocytosis, and normal actin organization, and contain larger and depolarized cortical actin patches and a prevalence of G-actin bars, also known as monomeric actin (6, 3). Cells expressing truncated Scd5p (Scd5p-delta338) accumulate an internal pool of fully glycosylated Suc2p invertase and mature alpha-factor (1). This phenotype was originally interpreted as a problem with protein secretion, but was later shown to be caused by defects in receptor-mediated endocytosis, similar to that seen in clathrin mutants (1, 6). Overexpression of SCD5 partially suppresses the actin defects of both chc1 and clc1 nulls (6).

Last updated: 2005-10-18 Contact SGD

References cited on this page View Complete Literature Guide for SCD5
1) Nelson KK, et al.  (1996) SCD5, a suppressor of clathrin deficiency, encodes a novel protein with a late secretory function in yeast. Mol Biol Cell 7(2):245-60
2) Parle-McDermott AG, et al.  (1996) Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I. Yeast 12(10B Suppl):999-1004
3) Henry KR, et al.  (2003) The actin-regulating kinase Prk1p negatively regulates Scd5p, a suppressor of clathrin deficiency, in actin organization and endocytosis. Curr Biol 13(17):1564-9
4) Chang JS, et al.  (2006) Cortical recruitment and nuclear-cytoplasmic shuttling of Scd5p, a protein phosphatase-1-targeting protein involved in actin organization and endocytosis. Mol Biol Cell 17(1):251-62
5) Nelson KK and Lemmon SK  (1993) Suppressors of clathrin deficiency: overexpression of ubiquitin rescues lethal strains of clathrin-deficient Saccharomyces cerevisiae. Mol Cell Biol 13(1):521-32
6) Henry KR, et al.  (2002) Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast. Mol Biol Cell 13(8):2607-25
7) Huang B, et al.  (2003) Identification of novel recognition motifs and regulatory targets for the yeast actin-regulating kinase Prk1p. Mol Biol Cell 14(12):4871-84
8) Chang JS, et al.  (2002) Protein phosphatase-1 binding to scd5p is important for regulation of actin organization and endocytosis in yeast. J Biol Chem 277(50):48002-8