Standard Name	LDB19 1
Systematic Name	YOR322C
Alias	ART1 2
Feature Type	ORF, Verified
Description	Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye (1, 2, 3)
Name Description	Low Dye Binding 1

Chromosomal Location	ChrXV:921062 to 918606 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for LDB19
Molecular Function
Manually curated	ubiquitin protein ligase binding (IPI)
Biological Process
Manually curated	ubiquitin-dependent endocytosis (IMP, IPI)
Cellular Component
Manually curated	cytosol (IDA) Golgi apparatus (IDA) plasma membrane (IDA)
High-throughput	colocalizes_with clathrin-coated vesicle (IDA) cytoplasm (IDA)

Classical genetics
null	tellurium atom accumulation: increased endocytosis: decreased metal resistance: decreased osmotic stress resistance: decreased Ptr2p-GFP accumulation: increased resistance to L-canavanine: decreased resistance to canavanine: decreased resistance to caffeine: decreased viable
reduction of function	endocytosis: decreased
Large-scale survey
null	auxotrophy chitin deposition: increased competitive fitness: decreased dessication resistance: decreased filamentous growth: increased haploinsufficient heat sensitivity: increased hyperosmotic stress resistance: decreased invasive growth: absent metal resistance: decreased nutrient utilization: increased oxidative stress resistance: decreased resistance to mycophenolic acid: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to caffeine: decreased resistance to acetic acid: decreased resistance to sulfometuron methyl: decreased resistance to sirolimus: decreased resistance to arsenite(3-): decreased resistance to ethanol: decreased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to methyl caffeate: decreased resistance to cycloheximide: decreased resistance to beta-chloro-L-alanine: decreased resistance to ethylenediaminetetraacetic acid: decreased resistance to 6-dimethylaminopurine: decreased resistance to cerulenin: decreased resistance to cadmium dichloride: decreased resistance to sodium selenide: decreased resistance to dimethyl sulfoxide: decreased resistance to glycolaldehyde: decreased sporulation efficiency: increased starvation resistance: decreased stress resistance: decreased telomere length: decreased toxin resistance: increased toxin resistance: decreased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	56 total interaction(s) for 48 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Biochemical Activity: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 22 Phenotypic Suppression: 1 Positive Genetic: 5 Synthetic Growth Defect: 11 Synthetic Lethality: 5 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:921062 to 918606 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2457 921062..918606 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005849