ISW2/YOR304W Summary

ISW2 BASIC INFORMATION

Standard Name	ISW2
Systematic Name	YOR304W
Feature Type	ORF, Verified
Description	ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth (1, 2, 3, 4)
Name Description	Imitation SWitch subfamily 1

GO Annotations	All ISW2 GO evidence and references
	View Computational GO annotations for ISW2
Molecular Function
Manually curated	DNA translocase activity (IDA) single-stranded DNA binding (IDA)
Biological Process
Manually curated	chromatin remodeling (IGI, IMP, IPI) chromatin silencing at rDNA (IMP) NOT chromatin silencing at telomere (IMP) chromatin silencing at telomere (IMP) negative regulation of antisense RNA transcription (IGI) negative regulation of transcription from RNA polymerase II promoter by pheromones (IMP) nucleosome positioning (IDA)
Cellular Component
Manually curated	chromatin accessibility complex (IPI, ISS) nucleus (IPI)

Mutant Phenotype	All ISW2 Phenotype details and references
Classical genetics
null	cell shape: abnormal heat sensitivity: normal invasive growth: increased resistance to L-1,4-dithiothreitol: decreased resistance to tunicamycin: normal resistance to mercaptoethanol: decreased viable
Large-scale survey
null	viable

Interactions	ISW2 All interactions details and references
	132 total interaction(s) for 89 unique genes/features.
Physical Interactions	Affinity Capture-MS: 43 Affinity Capture-Western: 6 Co-localization: 4 Reconstituted Complex: 1
Genetic Interactions	Phenotypic Enhancement: 46 Phenotypic Suppression: 10 Synthetic Growth Defect: 17 Synthetic Lethality: 3 Synthetic Rescue: 2

Sequence Information

ChrXV:884512 to 887874 | |

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3363	884512..887874	2006-01-05	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005831

ISW2 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ISW2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ISW2]

1)	Tsukiyama T, et al. (1999) Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev 13(6):686-97
2)	Gelbart ME, et al. (2001) Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates. Mol Cell Biol 21(6):2098-106
3)	Ruiz C, et al. (2003) Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex. Microbiology 149(Pt 2):341-51
4)	Fischer CJ, et al. (2009) Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2. Biochemistry 48(13):2960-8

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.