RPT4/YOR259C Summary Help

Standard Name RPT4 1, 2
Systematic Name YOR259C
Alias CRL13 , PCS1 , SUG2
Feature Type ORF, Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization (3, 4, 5, 6, 7)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Chromosomal Location
ChrXV:813708 to 812395 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 143 cM
Gene Ontology Annotations All RPT4 GO evidence and references
  View Computational GO annotations for RPT4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
250 total interaction(s) for 146 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 96
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 23
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 5
  • PCA: 6
  • Reconstituted Complex: 5
  • Two-hybrid: 25

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 68
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 437
Molecular Weight (Da) 49,408
Isoelectric Point (pI) 5.54
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:813708 to 812395 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 143 cM
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1314 813708..812395 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005785
References cited on this page View Complete Literature Guide for RPT4
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) McCusker, J.H. and J.E. Haber  (1996) SUG2 {crl13} is identical to CRL13. Personal Communication
3) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4) McDonald HB and Byers B  (1997) A proteasome cap subunit required for spindle pole body duplication in yeast. J Cell Biol 137(3):539-53
5) Russell SJ, et al.  (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
6) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7) Lipson C, et al.  (2008) A proteasomal ATPase contributes to dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates. J Biol Chem 283(11):7166-75