RPT4/YOR259C Summary

RPT4 BASIC INFORMATION

Standard Name	RPT4 1, 2
Systematic Name	YOR259C
Alias	CRL13 , PCS1 , SUG2
Feature Type	ORF, Verified
Description	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for spindle pole body duplication; localized mainly to the nucleus throughout the cell cycle (3, 4, 5, 6)
Name Description	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1

GO Annotations	All RPT4 GO evidence and references
	View Computational GO annotations for RPT4
Molecular Function
Manually curated	ATPase activity (IDA, TAS) endopeptidase activity (TAS)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	nucleus (IDA) proteasome regulatory particle, base subcomplex (IDA, IPI)

Mutant Phenotype	All RPT4 Phenotype details and references
Classical genetics
unspecified	Ub-Pro-beta-gal accumulation: increased
Large-scale survey
null	inviable resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased
repressible	mitochondrial morphology: abnormal

Interactions	RPT4 All interactions details and references
	123 total interaction(s) for 53 unique genes/features.
Physical Interactions	Affinity Capture-MS: 74 Affinity Capture-RNA: 1 Affinity Capture-Western: 17 Co-fractionation: 2 Co-localization: 1 Co-purification: 4 PCA: 4 Reconstituted Complex: 3 Two-hybrid: 7
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Synthetic Growth Defect: 3 Synthetic Rescue: 1

Sequence Information

ChrXV:813708 to 812395 | |

Note: this feature is encoded on the Crick strand.

Genetic position: 143 cM

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1314	813708..812395	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005785

RPT4 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RPT4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPT4]

1)	Finley D, et al. (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2)	McCusker, J.H. and J.E. Haber (1996) SUG2 {crl13} is identical to CRL13. Personal Communication
3)	Glickman MH, et al. (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4)	McDonald HB and Byers B (1997) A proteasome cap subunit required for spindle pole body duplication in yeast. J Cell Biol 137(3):539-53
5)	Russell SJ, et al. (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
6)	Rubin DM, et al. (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19

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