ISU2/YOR226C Summary Help

Standard Name ISU2 1
Systematic Name YOR226C
Alias NUA2
Feature Type ORF, Verified
Description Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved; ISU2 has a paralog, ISU1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description IscU homolog 1
Chromosomal Location
ChrXV:762084 to 761614 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ISU2 GO evidence and references
  View Computational GO annotations for ISU2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 16 genes
Classical genetics
Large-scale survey
27 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Protein-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 22
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 156
Molecular Weight (Da) 16,930
Isoelectric Point (pI) 10.34
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:762084 to 761614 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..471 762084..761614 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005752
References cited on this page View Complete Literature Guide for ISU2
1) Schilke B, et al.  (1999) Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(18):10206-11
2) Garland SA, et al.  (1999) Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly. J Mol Biol 294(4):897-907
3) Gerber J, et al.  (2004) The yeast scaffold proteins Isu1p and Isu2p are required inside mitochondria for maturation of cytosolic Fe/S proteins. Mol Cell Biol 24(11):4848-57
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76