HIS3/YOR202W Summary

Standard Name	HIS3 1
Systematic Name	YOR202W
Alias	HIS10 2 , HIS8 2
Feature Type	ORF, Verified
Description	Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	HIStidine 1

Chromosomal Location	ChrXV:721946 to 722608 \| ORF Map \| GBrowse

	Genetic position: 112 cM

Gene Ontology Annotations All HIS3 GO evidence and references

	View Computational GO annotations for HIS3
Molecular Function
Manually curated	imidazoleglycerol-phosphate dehydratase activity (IDA, IMP)
Biological Process
Manually curated	histidine biosynthetic process (IDA, IMP)
Cellular Component
Manually curated	cellular_component unknown (ND)

Pathways	histidine biosynthesis superpathway of histidine, purine, and pyrimidine biosynthesis

Mutant phenotypes All HIS3 Phenotype evidence and references

Classical genetics
null	auxotrophy
unspecified	imidzaole glycerol accumulation: increased metal resistance: decreased
Large-scale survey
null	resistance to amitrole: decreased resistance to cycloheximide: decreased viable
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HIS3 Interaction evidence and references

	39 total interaction(s) for 39 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 3 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 1 Phenotypic Enhancement: 10 Phenotypic Suppression: 12 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HIS3 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:721946 to 722608 | |

: 112 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..663	721946..722608	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005728

SUMMARY PARAGRAPH for HIS3

HIS3 encodes imidazoleglycerol-phosphate dehydratase, which catalyzes the sixth step in histidine biosynthesis (5). The biosynthesis of histidine has been most extensively studied in Salmonella typhimurium and E. coli. The reactions and enzymes involved in histidine biosynthesis have been identified in many organisms, and are thoroughly reviewed in Alifano et al. (5). Mutations in HIS3, as well as in genes encoding other histidine biosynthetic enzymes, cause histidine auxotrophy and sensitivity to copper, cobalt, and nickel salts (4). Transcription of HIS3 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role (reviewed in 6). HIS3 has been widely used in studies of transcriptional regulation (6) and as a selectable marker for plasmid constructs (for example, see 7 and 8).

Last updated: 1999-11-10

References cited on this page View Complete Literature Guide for HIS3

1)	Struhl K and Davis RW (1977) Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3). Proc Natl Acad Sci U S A 74(12):5255-9
2)	Fink GR (1964) GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. Science 146(3643):525-7
3)	Hope IA and Struhl K (1985) GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast. Cell 43(1):177-88
4)	Pearce DA and Sherman F (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9
5)	Alifano P, et al. (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69
6)	Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7)	Baganz F, et al. (1997) Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast 13(16):1563-73
8)	Sikorski RS and Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122(1):19-27