MCA1/YOR197W Summary Help

Standard Name MCA1 1
Systematic Name YOR197W
Alias YCA1 2
Feature Type ORF, Verified
Description Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization (2, 3, 4, 5)
Name Description MetaCAspase 1
Chromosomal Location
ChrXV:717086 to 718384 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MCA1 GO evidence and references
  View Computational GO annotations for MCA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 80 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
62 total interaction(s) for 54 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 5
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 432
Molecular Weight (Da) 47,982
Isoelectric Point (pI) 5.09
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:717086 to 718384 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2006-05-11
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1299 717086..718384 2011-02-03 2006-05-11
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005723
References cited on this page View Complete Literature Guide for MCA1
1) Szallies A, et al.  (2002) A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae. FEBS Lett 517(1-3):144-50
2) Madeo F, et al.  (2002) A caspase-related protease regulates apoptosis in yeast. Mol Cell 9(4):911-7
3) Lee RE, et al.  (2008) A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics. PLoS ONE 3(8):e2956
4) Lee RE, et al.  (2010) Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A 107(30):13348-53
5) Wong AH, et al.  (2012) Crystal structure of the yeast metacaspase Yca1. J Biol Chem 287(35):29251-9