DDP1/YOR163W Summary

Standard Name	DDP1 1
Systematic Name	YOR163W
Feature Type	ORF, Verified
Description	Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description	Diadenosine and Diphosphoinositol Polyphosphate phosphohydrolase

Chromosomal Location	ChrXV:642741 to 643307 \| ORF Map \| GBrowse

Gene Ontology Annotations All DDP1 GO evidence and references

	View Computational GO annotations for DDP1
Molecular Function
Manually curated	bis(5'-adenosyl)-hexaphosphatase activity (IDA) bis(5'-adenosyl)-pentaphosphatase activity (IDA) diphosphoinositol-polyphosphate diphosphatase activity (IDA) endopolyphosphatase activity (IDA) m7G(5')pppN diphosphatase activity (IDA)
Biological Process
Manually curated	diadenosine polyphosphate catabolic process (IDA) inositol phosphate dephosphorylation (IDA) polyphosphate catabolic process (IDA)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	inositol phosphate biosynthesis

Mutant phenotypes All DDP1 Phenotype evidence and references

Classical genetics
null	diphosphorylinositol pentakisphosphate accumulation: increased Pho4p-GFP distribution: abnormal viable
Large-scale survey
null	competitive fitness: increased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All DDP1 Interaction evidence and references

	21 total interaction(s) for 21 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 3 Biochemical Activity: 1 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 7 Positive Genetic: 4 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All DDP1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:642741 to 643307 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..567	642741..643307	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005689

SUMMARY PARAGRAPH for DDP1

DDP1 encodes a polyphosphate phosphatase that hydrolyzes inositol pyrophosphates (PP-IPs) and diadenosine polyphosphates (ApAs) (3, 2). PP-IPs and ApAs are metabolically unrelated classes of signaling molecules that are important for processes such as vacuolar biogenesis, DNA repair, cell wall synthesis, telomere maintenance, phosphate homeostasis, stress responses, cell proliferation, and ion-channel function (reviewed in 1 and 3 and references therein). As a PP-IP phosphatase, Ddp1p dephosphorylates both bis-diphosphoinositol tetrakisphosphate ([PP]2-IP4, IP8) and diphosphoinositol pentakisphosphate (PP-IP5, IP7) down to inositol hexakisphosphate (IP6) (3). As an ApA phosphatase, Ddp1p dephosphorylates diadenosine hexaphosphate (Ap6A) most readily, but can also dephosphorylate diadenosine pentaphosphate (Ap5A), adenosine pentaphosphate (p5A), and adenosine tetraphosphate (p4A) (2).

Ddp1 is a member of the MutT motif (nudix hydrolase) family of enzymes that is conserved from yeast to human (2). Homologs include fission yeast APS1 and human DIPP (2, 3 and references therein).

Last updated: 2008-04-30

References cited on this page View Complete Literature Guide for DDP1

1)	York JD (2006) Regulation of nuclear processes by inositol polyphosphates. Biochim Biophys Acta 1761(5-6):552-9
2)	Cartwright JL and McLennan AG (1999) The Saccharomyces cerevisiae YOR163w gene encodes a diadenosine 5', 5"'-P1,P6-hexaphosphate (Ap6A) hydrolase member of the MutT motif (Nudix hydrolase) family. J Biol Chem 274(13):8604-10
3)	Safrany ST, et al. (1999) The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein. J Biol Chem 274(31):21735-40
4)	Lonetti A, et al. (2011) Identification of an evolutionarily conserved family of inorganic polyphosphate endopolyphosphatases. J Biol Chem 286(37):31966-74
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Song MG, et al. (2013) Multiple Nudix family proteins possess mRNA decapping activity. RNA 19(3):390-9