ELG1/YOR144C Summary

Standard Name	ELG1 1
Systematic Name	YOR144C
Alias	RTT110
Feature Type	ORF, Verified
Description	Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description	Enhanced Level of Genomic instability 1

Chromosomal Location	ChrXV:605092 to 602717 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All ELG1 GO evidence and references

	View Computational GO annotations for ELG1
Molecular Function
Manually curated	chromatin binding (IDA)
Biological Process
Manually curated	DNA-dependent DNA replication (IMP) double-strand break repair via homologous recombination (IMP) mitotic sister chromatid cohesion (IGI, IMP) negative regulation of DNA recombination (NAS) negative regulation of transposition, RNA-mediated (IMP) telomere maintenance (TAS)
Cellular Component
Manually curated	Elg1 RFC-like complex (IPI)
High-throughput	cytoplasm (IDA) mitochondrion (IDA) nucleus (IDA)

Mutant phenotypes All ELG1 Phenotype evidence and references

Classical genetics
null	UV resistance: normal chromosome segregation: premature chromosome/plasmid maintenance: abnormal chromosome/plasmid maintenance: decreased colony sectoring: increased mitotic recombination: increased mutation frequency: increased Ddc2p distribution: abnormal resistance to hydroxyurea: normal resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased transposable element transposition: increased
Large-scale survey
null	bud morphology: abnormal competitive fitness: decreased mitotic recombination: increased Rad52-YFP distribution: increased resistance to camptothecin: increased resistance to cisplatin: increased resistance to caffeine: increased resistance to tunicamycin: increased resistance to amiodarone: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to sodium selenide: decreased resistance to doxorubicin: decreased telomere length: increased transposable element transposition: increased viable
overexpression	resistance to sirolimus: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ELG1 Interaction evidence and references

	551 total interaction(s) for 240 unique genes/features.
Physical Interactions	Affinity Capture-MS: 16 Affinity Capture-Western: 20 Reconstituted Complex: 6 Two-hybrid: 12
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 137 Phenotypic Enhancement: 25 Phenotypic Suppression: 9 Positive Genetic: 6 Synthetic Growth Defect: 165 Synthetic Lethality: 139 Synthetic Rescue: 12
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ELG1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:605092 to 602717 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2376	605092..602717	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005670

SUMMARY PARAGRAPH for ELG1

Elg1p is a conserved but uncharacterized homolog of the large replication factor C (RFC) subunit Rfc1p and the alternative RFC subunits Ctf18p and Rad24p (3). Elg1p forms an alternative RFC complex with the Rfc2p, Rfc3p, Rfc4p, and Rfc5p subunits of RFC that is distinct from the previously described RFC-like complexes containing Rad24p and Ctf18p (1). This alternative Elg1p-RFC complex is important for DNA replication and genome integrity (2).

Elg1p acts in concert with Ctf18p and Rad24p to enable Rad53p checkpoint kinase activation in response to replication stress (3). Genetic data indicate that the Elg1p, Ctf18p, and Rad24p RFC-like complexes work in three separate pathways important for maintaining the integrity of the genome (1). Further, Elg1p interacts physically with Pol30p and Rad27p, has a possible role in Okazaki fragment maturation, and is required for efficient S phase progression and telomere homeostasis (3).

Strains mutant in Elg1p are defective in recovery from DNA damage during S phase, display DNA replication defects, exhibit elevated levels of recombination between homologous and nonhomologous chromosomes, sister chromatids, and direct repeats, and show increased levels of chromosome loss (1, 2).

Last updated: 2004-04-15

References cited on this page View Complete Literature Guide for ELG1

1)	Ben-Aroya S, et al. (2003) ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C. Proc Natl Acad Sci U S A 100(17):9906-11
2)	Bellaoui M, et al. (2003) Elg1 forms an alternative RFC complex important for DNA replication and genome integrity. EMBO J 22(16):4304-13
3)	Kanellis P, et al. (2003) Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol 13(18):1583-95
4)	Ogiwara H, et al. (2007) Role of Elg1 protein in double strand break repair. Nucleic Acids Res 35(2):353-62
5)	Davidson MB and Brown GW (2008) The N- and C-termini of Elg1 contribute to the maintenance of genome stability. DNA Repair (Amst) 7(8):1221-32
6)	Kubota T, et al. (2013) The Elg1 Replication Factor C-like Complex Functions in PCNA Unloading during DNA Replication. Mol Cell 50(2):273-80