ELG1/YOR144C Summary Help

Standard Name ELG1 1
Systematic Name YOR144C
Alias RTT110
Feature Type ORF, Verified
Description Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Enhanced Level of Genomic instability 1
Chromosomal Location
ChrXV:605092 to 602717 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ELG1 GO evidence and references
  View Computational GO annotations for ELG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
560 total interaction(s) for 243 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-Western: 20
  • Reconstituted Complex: 6
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 137
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 9
  • Positive Genetic: 7
  • Synthetic Growth Defect: 168
  • Synthetic Lethality: 139
  • Synthetic Rescue: 14

Resources
Expression Summary
histogram
Resources
Length (a.a.) 791
Molecular Weight (Da) 91,261
Isoelectric Point (pI) 6.98
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:605092 to 602717 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2376 605092..602717 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005670
SUMMARY PARAGRAPH for ELG1

Elg1p is a conserved but uncharacterized homolog of the large replication factor C (RFC) subunit Rfc1p and the alternative RFC subunits Ctf18p and Rad24p (3). Elg1p forms an alternative RFC complex with the Rfc2p, Rfc3p, Rfc4p, and Rfc5p subunits of RFC that is distinct from the previously described RFC-like complexes containing Rad24p and Ctf18p (1). This alternative Elg1p-RFC complex is important for DNA replication and genome integrity (2).

Elg1p acts in concert with Ctf18p and Rad24p to enable Rad53p checkpoint kinase activation in response to replication stress (3). Genetic data indicate that the Elg1p, Ctf18p, and Rad24p RFC-like complexes work in three separate pathways important for maintaining the integrity of the genome (1). Further, Elg1p interacts physically with Pol30p and Rad27p, has a possible role in Okazaki fragment maturation, and is required for efficient S phase progression and telomere homeostasis (3).

Strains mutant in Elg1p are defective in recovery from DNA damage during S phase, display DNA replication defects, exhibit elevated levels of recombination between homologous and nonhomologous chromosomes, sister chromatids, and direct repeats, and show increased levels of chromosome loss (1, 2).

Last updated: 2004-04-15 Contact SGD

References cited on this page View Complete Literature Guide for ELG1
1) Ben-Aroya S, et al.  (2003) ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C. Proc Natl Acad Sci U S A 100(17):9906-11
2) Bellaoui M, et al.  (2003) Elg1 forms an alternative RFC complex important for DNA replication and genome integrity. EMBO J 22(16):4304-13
3) Kanellis P, et al.  (2003) Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol 13(18):1583-95
4) Ogiwara H, et al.  (2007) Role of Elg1 protein in double strand break repair. Nucleic Acids Res 35(2):353-62
5) Davidson MB and Brown GW  (2008) The N- and C-termini of Elg1 contribute to the maintenance of genome stability. DNA Repair (Amst) 7(8):1221-32
6) Kubota T, et al.  (2013) The Elg1 Replication Factor C-like Complex Functions in PCNA Unloading during DNA Replication. Mol Cell 50(2):273-80