ADE2/YOR128C Summary Help

Standard Name ADE2 1
Systematic Name YOR128C
Feature Type ORF, Verified
Description Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine (2 and see Summary Paragraph)
Name Description ADEnine requiring 1
Chromosomal Location
ChrXV:566191 to 564476 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 64 cM
Gene Ontology Annotations All ADE2 GO evidence and references
  View Computational GO annotations for ADE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
48 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 1
  • PCA: 2
  • Two-hybrid: 8

Genetic Interactions
  • Negative Genetic: 11
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 6
  • Positive Genetic: 1
  • Synthetic Growth Defect: 5
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 571
Molecular Weight (Da) 62,339
Isoelectric Point (pI) 7.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:566191 to 564476 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 64 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1716 566191..564476 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005654
SUMMARY PARAGRAPH for ADE2

The ADE2 gene encodes phosphoribosylaminoimidazole carboxylase, which catalyzes the sixth step in the de novo biosynthesis of purine nucleotides (pathway shown here; 2). ADE2 transcription is regulated specifically by adenine and also by general amino-acid control (3). Gene expression is repressed in the presence of adenine, activated in the absence of adenine, and also slightly increased in conditions of amino-acid starvation (4, 3, 5). Maximal ADE2 expression is dependent on the transcriptional activators Gcn4p, Bas1p, and Pho2p, which bind to 5'-TGACTC-3' sites in the ADE2 promoter (4, 5). Gcn4p upregulates ADE2 expression during amino acid starvation, a condition which inhibits Gcn4p degradation (6). Bas1p binds the ADE2 promoter in both repressing and derepressing growth conditions, but binding increases slightly when adenine is limiting. Under derepressing conditions, Pho2p also binds the ADE2 promoter in a Bas1p-dependent manner, and transcription is induced (5). Derepression by Bas1p and Pho2p also requires the purine biosynthesis pathway intermediates 5-phosphoribosyl-4-succinocarboxamide-5-aminoimidazole and 5-phosphoribosyl-4-carboxamide- 5-aminoimidazole, which are thought to promote interaction between these two activators (7, 5).

Mutations in ADE2 lead to the accumulation of purine precursors in the vacuole, which causes the colony to be red in color. This pigmentation phenotype is widely utilized as a marker for genetic selection and screening (2, 8 and references contained therein).

Last updated: 2005-12-21 Contact SGD

References cited on this page View Complete Literature Guide for ADE2
1) Dorfman BZ  (1969) The isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains. Genetics 61(2):377-89
2) Jones EW and Fink GR  (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
3) Gedvilaite A and Sasnauskas K  (1994) Control of the expression of the ADE2 gene of the yeast Saccharomyces cerevisiae. Curr Genet 25(6):475-9
4) Stotz A, et al.  (1993) Regulation of the ADE2 gene from Saccharomyces cerevisiae. Curr Genet 24(6):472-80
5) Som I, et al.  (2005) DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae. Eukaryot Cell 4(10):1725-35
6) Kornitzer D, et al.  (1994) Regulated degradation of the transcription factor Gcn4. EMBO J 13(24):6021-30
7) Rebora K, et al.  (2001) Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate. Mol Cell Biol 21(23):7901-12
8) Zonneveld BJ and van der Zanden AL  (1995) The red ade mutants of Kluyveromyces lactis and their classification by complementation with cloned ADE1 or ADE2 genes from Saccharomyces cerevisiae. Yeast 11(9):823-7