LEO1/YOR123C Summary

LEO1/YOR123C Summary

Standard Name	LEO1 1
Systematic Name	YOR123C
Feature Type	ORF, Verified
Description	Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay (2, 3, 4, 5, 6, 7)
Name Description	LEft Open reading frame 1

Chromosomal Location	ChrXV:554570 to 553176 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All LEO1 GO evidence and references

	View Computational GO annotations for LEO1
Molecular Function
Manually curated	RNA binding (IDA) RNA polymerase II transcription factor binding transcription factor activity (IPI)
Biological Process
Manually curated	chromatin organization (IMP) histone H3-K4 trimethylation (IMP) positive regulation of transcription elongation from RNA polymerase I promoter (IDA) positive regulation of transcription elongation from RNA polymerase II promoter (IMP) regulation of histone H2B conserved C-terminal lysine ubiquitination (IDA) regulation of transcription-coupled nucleotide-excision repair (IGI) transcription elongation from RNA polymerase II promoter (IGI)
Cellular Component
Manually curated	Cdc73/Paf1 complex (IPI) nucleus (IDA)

Mutant phenotypes All LEO1 Phenotype evidence and references

Classical genetics
unspecified	transposable element transposition: decreased
Large-scale survey
null	auxotrophy bud morphology: abnormal competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to cycloheximide: increased resistance to camptothecin: increased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to sodium arsenite: increased resistance to fluconazole: increased resistance to mycophenolic acid: decreased resistance to ethanol: decreased resistance to arsenite(3-): decreased resistance to methyl methanesulfonate: decreased resistance to bleomycin: decreased resistance to hydroxyurea: decreased resistance to BPS: decreased resistance to fenpropimorph: increased resistance to calcium dichloride: decreased sporulation: decreased sporulation efficiency: decreased stress resistance: decreased telomere length: decreased transposable element transposition: increased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All LEO1 Interaction evidence and references

	524 total interaction(s) for 272 unique genes/features.
Physical Interactions	Affinity Capture-MS: 70 Affinity Capture-RNA: 2 Affinity Capture-Western: 9 Biochemical Activity: 9 Co-fractionation: 1 Protein-RNA: 4 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 2 Negative Genetic: 280 Phenotypic Enhancement: 9 Phenotypic Suppression: 5 Positive Genetic: 47 Synthetic Growth Defect: 55 Synthetic Lethality: 20 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All LEO1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:554570 to 553176 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1395	554570..553176	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005649

References cited on this page View Complete Literature Guide for LEO1

1)	Magdolen V, et al. (1994) The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein. Biochim Biophys Acta 1218(2):205-9
2)	Mueller CL and Jaehning JA (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
3)	Betz JL, et al. (2002) Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism. Mol Genet Genomics 268(2):272-85
4)	Krogan NJ, et al. (2003) The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell 11(3):721-9
5)	Wood A, et al. (2003) The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. J Biol Chem 278(37):34739-42
6)	Nyswaner KM, et al. (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
7)	Tous C, et al. (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64