LEO1/YOR123C Summary

LEO1 BASIC INFORMATION

Standard Name	LEO1
Systematic Name	YOR123C
Feature Type	ORF, Verified
Description	Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition (1, 2, 3, 4, 5)

GO Annotations	All LEO1 GO evidence and references
	View Computational GO annotations for LEO1
Molecular Function
Manually curated	RNA polymerase II transcription elongation factor activity (IPI)
Biological Process
Manually curated	histone methylation (IMP) negative regulation of transposition, RNA-mediated (IMP) RNA elongation from RNA polymerase II promoter (IPI) transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	Cdc73/Paf1 complex (IPI) nucleus (IDA) transcription elongation factor complex (IPI)

Mutant Phenotype	All LEO1 Phenotype details and references
Classical genetics
unspecified	transposable element transposition: decreased
Large-scale survey
null	bud morphology: abnormal competitive fitness: decreased heat sensitivity: increased resistance to calcium dichloride: decreased resistance to mycophenolic acid: decreased resistance to ethanol: decreased sporulation: decreased telomere length: decreased transposable element transposition: increased viable
overexpression	resistance to rapamycin: increased

Interactions	LEO1 All interactions details and references
	305 total interaction(s) for 170 unique genes/features.
Physical Interactions	Affinity Capture-MS: 60 Affinity Capture-Western: 4 Biochemical Activity: 9 Co-fractionation: 1 Two-hybrid: 1
Genetic Interactions	Phenotypic Enhancement: 123 Phenotypic Suppression: 32 Synthetic Growth Defect: 30 Synthetic Lethality: 43 Synthetic Rescue: 2

Sequence Information

ChrXV:554571 to 553177 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1395	554571..553177	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005649

LEO1 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for LEO1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for LEO1]

1)	Mueller CL and Jaehning JA (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
2)	Betz JL, et al. (2002) Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism. Mol Genet Genomics 268(2):272-85
3)	Krogan NJ, et al. (2003) The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell 11(3):721-9
4)	Wood A, et al. (2003) The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. J Biol Chem 278(37):34739-42
5)	Nyswaner KM, et al. (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214

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