AZF1/YOR113W Summary

AZF1/YOR113W Summary

Standard Name	AZF1 1
Systematic Name	YOR113W
Feature Type	ORF, Verified
Description	Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7)
Name Description	Asparagine-rich Zinc-Finger 1

Chromosomal Location	ChrXV:534075 to 536819 \| ORF Map \| GBrowse

Gene Ontology Annotations All AZF1 GO evidence and references

	View Computational GO annotations for AZF1
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA, IMP) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA, IMP)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	cellular response to carbohydrate stimulus (IMP) regulation of fungal-type cell wall organization (IMP) regulation of transcription from RNA polymerase II promoter (IDA, IMP)
Cellular Component
Manually curated	cytosol (IDA) nucleus (IDA)

Regulatory Role

Binding motifs	Predicted AZF1 Binding Site Locations
	BTTCTTTTB 8 [Search intergenic regions for BTTCTTTTB] TTTTTCTT 3 [Search intergenic regions for TTTTTCTT] YWTTKCKKTYYCKGYKKY 9 [Search intergenic regions for YWTTKCKKTYYCKGYKKY]

Regulatory modules	predicted: stressResponse (571)

Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All AZF1 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased haploproficient resistance to nickel sulfate: increased vacuolar morphology: abnormal vegetative growth: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All AZF1 Interaction evidence and references

	71 total interaction(s) for 61 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 2 Biochemical Activity: 7 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 47 Positive Genetic: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All AZF1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:534075 to 536819 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2745	534075..536819	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005639

References cited on this page View Complete Literature Guide for AZF1

1)	Brohl S, et al. (1994) A new nuclear suppressor system for a mitochondrial RNA polymerase mutant identifies an unusual zinc-finger protein and a polyglutamine domain protein in Saccharomyces cerevisiae. Yeast 10(6):719-31
2)	Stein T, et al. (1998) Azf1p is a nuclear-localized zinc-finger protein that is preferentially expressed under non-fermentative growth conditions in Saccharomyces cerevisiae. Curr Genet 34(4):287-96
3)	Newcomb LL, et al. (2002) AZF1 is a glucose-dependent positive regulator of CLN3 transcription in Saccharomyces cerevisiae. Mol Cell Biol 22(5):1607-14
4)	Kucejova B and Foury F (2003) Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system. Folia Microbiol (Praha) 48(2):183-8
5)	Slattery MG, et al. (2006) The function and properties of the Azf1 transcriptional regulator change with growth conditions in Saccharomyces cerevisiae. Eukaryot Cell 5(2):313-20
6)	Ghosh Dastidar R, et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
7)	Mirzaei H, et al. (2013) Systematic measurement of transcription factor-DNA interactions by targeted mass spectrometry identifies candidate gene regulatory proteins. Proc Natl Acad Sci U S A 110(9):3645-50
8)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
9)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104