Standard Name	VAM3 1 (see Nomenclature conflict Note)
Systematic Name	YOR106W
Alias	PTH1
Feature Type	ORF, Verified
Description	Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region (1, 2, 3, 4, 5)
Name Description	VAcuolar Morphogenesis 1

Chromosomal Location	ChrXV:519121 to 519972 | ORF Map | GBrowse

	View Computational GO annotations for VAM3
Molecular Function
Manually curated	protein homodimerization activity (IDA) SNAP receptor activity (IDA, ISS)
Biological Process
Manually curated	autophagic vacuole fusion (IMP) piecemeal microautophagy of nucleus (IMP) vacuole fusion, non-autophagic (IDA) vesicle docking (IGI) vesicle fusion (IDA) vesicle fusion with vacuole (IGI)
Cellular Component
Manually curated	fungal-type vacuole membrane (IDA) SNARE complex (IDA)

Classical genetics
conditional	autophagy: abnormal
null	lithium(1+) accumulation: increased metal resistance: decreased GFP-Osh1p accumulation: increased Bud8p-GFP distribution: abnormal carboxypeptidase Y (Prc1p) modification: decreased replicative lifespan: decreased resistance to bortezomib: decreased resistance to caffeine: decreased resistance to ferrozine(2-): decreased resistance to cobalt chloride hexahydrate: decreased
Large-scale survey
null	UV resistance: decreased anaerobic growth: decreased auxotrophy budding pattern: abnormal cell shape: abnormal rubidium(1+) accumulation: decreased polyphosphate accumulation: abnormal glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased ionic stress resistance: decreased oxidative stress resistance: decreased Cps1p (CPS) modification: decreased Pho8p (ALP) modification: decreased resistance to sirolimus: decreased resistance to monensin: decreased resistance to PM02734: decreased resistance to ethanol: decreased resistance to methanol: decreased resistance to tirapazamine: decreased resistance to caffeine: decreased resistance to methyl methanesulfonate: decreased resistance to 4-nitroquinoline N-oxide: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to bleomycin: decreased resistance to fluconazole: decreased resistance to hydroxyurea: decreased resistance to mycophenolic acid: decreased resistance to dimethyl sulfoxide: decreased resistance to mefloquine: decreased resistance to clioquinol: decreased resistance to wortmannin: normal resistance to BPS: decreased sporulation: absent stress resistance: decreased vacuolar morphology: abnormal viable
overexpression	inviable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	375 total interaction(s) for 185 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 88 Co-fractionation: 15 Co-purification: 10 FRET: 1 Reconstituted Complex: 23 Two-hybrid: 14
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 2 Dosage Rescue: 8 Negative Genetic: 144 Phenotypic Enhancement: 12 Positive Genetic: 34 Synthetic Growth Defect: 10 Synthetic Lethality: 11
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:519121 to 519972 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..852 519121..519972 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005632