VPS5/YOR069W Summary Help

Standard Name VPS5 1, 2
Systematic Name YOR069W
Alias GRD2 , PEP10 , VPT5 3
Feature Type ORF, Verified
Description Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication (4, 5, 6, 7, 8)
Also known as: YOR29-20
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXV:453768 to 455795 | ORF Map | GBrowse
Gene Ontology Annotations All VPS5 GO evidence and references
  View Computational GO annotations for VPS5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 27 genes
Classical genetics
reduction of function
Large-scale survey
297 total interaction(s) for 196 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 12
  • Reconstituted Complex: 2
  • Two-hybrid: 19

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 166
  • Phenotypic Suppression: 1
  • Positive Genetic: 29
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 675
Molecular Weight (Da) 76,484
Isoelectric Point (pI) 4.93
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:453768 to 455795 | ORF Map | GBrowse
This feature contains embedded feature(s): VAM10
Last Update Coordinates: 2011-02-03 | Sequence: 2004-04-21
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2028 453768..455795 2011-02-03 2004-04-21
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005595
References cited on this page View Complete Literature Guide for VPS5
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Bankaitis VA, et al.  (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
4) Seaman MN, et al.  (1998) A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol 142(3):665-81
5) Nothwehr SF and Hindes AE  (1997) The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi. J Cell Sci 110 ( Pt 9)():1063-72
6) Reddy JV and Seaman MN  (2001) Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell 12(10):3242-56
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15