ASE1/YOR058C Summary Help

Standard Name ASE1 1
Systematic Name YOR058C
Alias YOR29-09
Feature Type ORF, Verified
Description Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress (2, 3, 4, 5)
Name Description Anaphase Spindle Elongation 1
Chromosomal Location
ChrXV:436345 to 433688 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ASE1 GO evidence and references
  View Computational GO annotations for ASE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
misexpression
null
overexpression
Large-scale survey
null
overexpression
Resources
244 total interaction(s) for 128 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Protein-RNA: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 116
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 8
  • Positive Genetic: 12
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 47
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 885
Molecular Weight (Da) 101,623
Isoelectric Point (pI) 9.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:436345 to 433688 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2658 436345..433688 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005584
References cited on this page View Complete Literature Guide for ASE1
1) Pellman D, et al.  (1995) Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae. J Cell Biol 130(6):1373-85
2) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
3) Schuyler SC, et al.  (2003) The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins. J Cell Biol 160(4):517-28
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76