DBP5/YOR046C Summary Help

Standard Name DBP5 1
Systematic Name YOR046C
Alias RAT8 2
Feature Type ORF, Verified
Description Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress (3, 4, 5, 6, 7)
Name Description Dead Box Protein 1
Chromosomal Location
ChrXV:415907 to 414459 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DBP5 GO evidence and references
  View Computational GO annotations for DBP5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
dominant negative
reduction of function
Large-scale survey
reduction of function
252 total interaction(s) for 181 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 19
  • Negative Genetic: 37
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 108
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 482
Molecular Weight (Da) 53,873
Isoelectric Point (pI) 7.48
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:415907 to 414459 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1449 415907..414459 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005572
References cited on this page View Complete Literature Guide for DBP5
1) Tseng SS, et al.  (1998) Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export. EMBO J 17(9):2651-62
2) Snay-Hodge CA, et al.  (1998) Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export. EMBO J 17(9):2663-76
3) Estruch F and Cole CN  (2003) An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components. Mol Biol Cell 14(4):1664-76
4) Tseng-Rogenski SS, et al.  (2003) Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes. Nucleic Acids Res 31(17):4995-5002
5) Gross T, et al.  (2007) The DEAD-box RNA helicase Dbp5 functions in translation termination. Science 315(5812):646-9
6) Noble KN, et al.  (2011) The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1. Genes Dev 25(10):1065-77
7) Tieg B and Krebber H  (2013) Dbp5 - from nuclear export to translation. Biochim Biophys Acta 1829(8):791-8