CUE5/YOR042W Summary Help

Standard Name CUE5 1
Systematic Name YOR042W
Feature Type ORF, Verified
Description Ubiquitin-binding protein; functions as a ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; contains a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; GFP-fusion protein localizes to the cytoplasm in a punctate pattern; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q (1, 2, 3, 4)
Name Description Coupling of Ubiquitin conjugation to ER degradation 5
Chromosomal Location
ChrXV:408425 to 409660 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CUE5 GO evidence and references
  View Computational GO annotations for CUE5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
67 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 3
  • Co-purification: 1
  • PCA: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 20
  • Phenotypic Enhancement: 1
  • Positive Genetic: 14
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 411
Molecular Weight (Da) 46,869
Isoelectric Point (pI) 4.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:408425 to 409660 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1236 408425..409660 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005568
References cited on this page View Complete Literature Guide for CUE5
1) Shih SC, et al.  (2003) A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J 22(6):1273-81
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Lu K, et al.  (2014) Autophagic Clearance of PolyQ Proteins Mediated by Ubiquitin-Atg8 Adaptors of the Conserved CUET Protein Family. Cell 158(3):549-63
5) Biederer T, et al.  (1997) Role of Cue1p in ubiquitination and degradation at the ER surface. Science 278(5344):1806-9