HIR2/YOR038C Summary Help

Standard Name HIR2 1
Systematic Name YOR038C
Alias SPT1 2
Feature Type ORF, Verified
Description Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia (1, 3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description HIstone Regulation 1, 10
Chromosomal Location
ChrXV:405388 to 402761 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HIR2 GO evidence and references
  View Computational GO annotations for HIR2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 6 genes
Regulators 2 genes
Classical genetics
Large-scale survey
398 total interaction(s) for 245 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 17
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 232
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 9
  • Positive Genetic: 52
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 10
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 875
Molecular Weight (Da) 98,444
Isoelectric Point (pI) 8.41
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:405388 to 402761 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2628 405388..402761 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005564

Hir2p is a nonessential corepressor of histone gene transcription; like other corepressors, it appears not to directly contact DNA, but can repress transcription when artificially recruited to yeast promoters (6, 3, 10). Hir2p and corepressor Hir1p help control the cell cycle regulation of transcription of histone genes HTA1, HTB1, HHT1, HHT2, HHF1, and HHF2 (1). Hir1p and Hir2p coimmunoprecipitate from yeast cell extracts, and may act as a complex (6). Hir2p has a nuclear targeting signal and is localized in the nucleus (1). The human disease called DiGeorge Syndrome may be caused by the loss of one copy of the HIRA gene, which contains features present in yeast HIR1 and HIR2 (11).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for HIR2
1) Sherwood PW, et al.  (1993) Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol 13(1):28-38
2) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
3) DeSilva H, et al.  (1998) Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor. Genetics 148(2):657-67
4) Kaufman PD, et al.  (1998) Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I. Mol Cell Biol 18(8):4793-806
5) Dimova D, et al.  (1999) A role for transcriptional repressors in targeting the yeast Swi/Snf complex. Mol Cell 4(1):75-83
6) Spector MS, et al.  (1997) Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle. Mol Cell Biol 17(2):545-52
7) Sharp JA, et al.  (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73
8) Prochasson P, et al.  (2005) The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Genes Dev 19(21):2534-9
9) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
10) Osley MA and Lycan D  (1987) Trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes. Mol Cell Biol 7(12):4204-10
11) Lamour V, et al.  (1995) A human homolog of the S. cerevisiae HIR1 and HIR2 transcriptional repressors cloned from the DiGeorge syndrome critical region. Hum Mol Genet 4(5):791-9