PEP12/YOR036W Summary Help

PEP12 BASIC INFORMATION

Standard Name PEP12 1
Systematic Name YOR036W
Alias VPL6 2 , VPS6 3 , 4 , VPT13 3
Feature Type ORF, Verified
Description Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin (5, 6, 7, 8)
Name Description carboxyPEPtidase Y-deficient 9
GO Annotations All PEP12 GO evidence and references
    View Computational GO annotations for PEP12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All PEP12 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Interactions PEP12 All interactions details and references
79 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 12
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Phenotypic Enhancement: 12
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2
Sequence Information
ChrXV:400348 to 401214 | ORF Map | GBrowse
Gbrowse
Genetic position: -5 cM
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..867 400348..401214 2006-01-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005562

PEP12 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for PEP12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PEP12]

1) Jones, E.  (1985) Personal Communication, Mortimer Map Edition 9
2) Rothman JH and Stevens TH  (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
3) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
4) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
5) Bassham DC, et al.  (1995) An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant. Proc Natl Acad Sci U S A 92(16):7262-6
6) Becherer KA, et al.  (1996) Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast. Mol Biol Cell 7(4):579-94
7) Gotte M and Gallwitz D  (1997) High expression of the yeast syntaxin-related Vam3 protein suppresses the protein transport defects of a pep12 null mutant. FEBS Lett 411(1):48-52
8) Gerrard SR, et al.  (2000) Pep12p is a multifunctional yeast syntaxin that controls entry of biosynthetic, endocytic and retrograde traffic into the prevacuolar compartment. Traffic 1(3):259-69
9) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33