DFG16/YOR030W Summary Help

Standard Name DFG16 1
Systematic Name YOR030W
Alias ECM41 , ZRG12 2
Feature Type ORF, Verified
Description Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p (1, 3, 4)
Name Description Defective for Filamentous Growth 5
Chromosomal Location
ChrXV:386825 to 388684 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DFG16 GO evidence and references
  View Computational GO annotations for DFG16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
238 total interaction(s) for 170 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 173
  • Phenotypic Suppression: 4
  • Positive Genetic: 30
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 619
Molecular Weight (Da) 71,729
Isoelectric Point (pI) 10.15
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:386825 to 388684 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1860 386825..388684 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005556
References cited on this page View Complete Literature Guide for DFG16
1) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) Barwell KJ, et al.  (2005) Relationship of DFG16 to the Rim101p pH response pathway in Saccharomyces cerevisiae and Candida albicans. Eukaryot Cell 4(5):890-9
4) Obara K, et al.  (2012) Membrane protein Rim21 plays a central role in sensing ambient pH in Saccharomyces cerevisiae. J Biol Chem 287(46):38473-81
5) Mosch HU and Fink GR  (1997) Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics 145(3):671-84