AUS1/YOR011W Summary Help

Standard Name AUS1 1
Systematic Name YOR011W
Feature Type ORF, Verified
Description Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description ABC protein involved in Uptake of Sterols 1
Chromosomal Location
ChrXV:349679 to 353863 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All AUS1 GO evidence and references
  View Computational GO annotations for AUS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
62 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • PCA: 21
  • Protein-peptide: 18
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,394
Molecular Weight (Da) 157,750
Isoelectric Point (pI) 7.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:349679 to 353863 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4185 349679..353863 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000005537
References cited on this page View Complete Literature Guide for AUS1
1) Wilcox LJ, et al.  (2002) Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast. J Biol Chem 277(36):32466-72
2) Alimardani P, et al.  (2004) SUT1-promoted sterol uptake involves the ABC transporter Aus1 and the mannoprotein Dan1 whose synergistic action is sufficient for this process. Biochem J 381(Pt 1):195-202
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Kohut P, et al.  (2011) The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast: Dehydroergosterol fluorescence study. Biochem Biophys Res Commun 404(1):233-8
5) Marek M, et al.  (2011) The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity. J Biol Chem 286(24):21835-43