PSF3/YOL146W Summary Help

Standard Name PSF3 1
Systematic Name YOL146W
Feature Type ORF, Verified
Description Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery (1, 2 and see Summary Paragraph)
Name Description Partner of Sld Five 1
Chromosomal Location
ChrXV:48864 to 49448 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PSF3 GO evidence and references
  View Computational GO annotations for PSF3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
Resources
49 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-purification: 1
  • Reconstituted Complex: 19
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 4
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 194
Molecular Weight (Da) 21,952
Isoelectric Point (pI) 5.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:48864 to 49448 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..585 48864..49448 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005506
SUMMARY PARAGRAPH for PSF3

The GINS complex (named for Go, Ichi, Nii, and San for five, one, two, and three in Japanese) is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together (1). The GINS complex was independently isolated in a large scale screen for cell cycle defects (2). A similar complex is found in Xenopus and has a ring-like structure (3). In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA (1, 2). The complex localizes to origins of DNA replication (1, 2) and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11 (4). Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).

Last updated: 2005-12-02 Contact SGD

References cited on this page View Complete Literature Guide for PSF3
1) Takayama Y, et al.  (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65
2) Kanemaki M, et al.  (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 423(6941):720-4
3) Kubota Y, et al.  (2003) A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication. Genes Dev 17(9):1141-52
4) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9