Standard Name	RIB4 1
Systematic Name	YOL143C
Feature Type	ORF, Verified
Description	Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin (2, 3)
Name Description	RIBoflavin biosynthesis 1

Chromosomal Location	ChrXV:55104 to 54595 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for RIB4
Molecular Function
Manually curated	6,7-dimethyl-8-ribityllumazine synthase activity (IGI, ISS)
Biological Process
Manually curated	riboflavin biosynthetic process (IMP)
Cellular Component
Manually curated	mitochondrial intermembrane space (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	riboflavin, FMN and FAD biosynthesis

Classical genetics
null	metal resistance: decreased resistance to selenium(2+): decreased
unspecified	auxotrophy 6,7-dimethyl-8-ribityllumazine accumulation: increased
Large-scale survey
null	acid pH resistance: decreased alkaline pH resistance: decreased budding pattern: abnormal cell shape: abnormal fermentative growth: decreased rate heat sensitivity: increased metal resistance: decreased resistance to cyclosporin A: decreased resistance to pentachlorophenol: decreased resistance to cadmium dichloride: decreased resistance to acetic acid: decreased resistance to ethanol: decreased resistance to sodium chloride: decreased resistance to sorbic acid: decreased resistance to calcofluor white: decreased resistance to oxalic acid: decreased resistance to propionic acid: decreased respiratory growth: absent starvation resistance: decreased utilization of nitrogen source: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	filamentous growth: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	22 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 4 Two-hybrid: 5
Genetic Interactions	Synthetic Growth Defect: 7 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:55104 to 54595 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..510 55104..54595 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005503