ARG8/YOL140W Summary

Standard Name	ARG8
Systematic Name	YOL140W
Feature Type	ORF, Verified
Description	Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine (1 and see Summary Paragraph)
Name Description	ARGinine requiring

Chromosomal Location	ChrXV:58759 to 60030 \| ORF Map \| GBrowse

	Genetic position: -130 cM

Gene Ontology Annotations All ARG8 GO evidence and references

	View Computational GO annotations for ARG8
Molecular Function
Manually curated	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity (IDA)
Biological Process
Manually curated	arginine biosynthetic process (TAS) ornithine biosynthetic process (TAS)
Cellular Component
Manually curated	mitochondrial matrix (IDA)
High-throughput	mitochondrion (IDA)

Pathways	arginine biosynthesis

interactions All ARG8 Interaction evidence and references

	6 total interaction(s) for 6 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Two-hybrid: 1
Genetic Interactions	Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ARG8 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:58759 to 60030 | |

: -130 cM

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1272	58759..60030	2006-01-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005500

SUMMARY PARAGRAPH for ARG8

ARG8 encodes acetylornithine aminotransferase, a mitochondrial matrix enzyme that catalyzes the fourth step in the biosynthesis of ornithine (2), an intermediate in arginine biosynthesis. Arg8p is 68% identical to the acetylornithine aminotransferase from Kluyveromyces lactis, and the K. lactis gene can complement an S. cerevisiae arg8 mutant (3). Arg8p is also similar to E. coli ArgD (1). Like other genes encoding arginine biosynthetic enzymes, ARG8 is transcriptionally repressed in the presence of arginine and is regulated by general amino acid control (4, 5). Arginine-responsive transcription factors, including Arg80p, Arg81p, Arg82p, and Mcm1p, and their target upstream activating sequences in ARG8, have been identified (4, 6, 7, 5). The ARG8 sequence has been recoded using the mitochondrial genetic code, for use as a marker for mitochondrial transformation (8). A construct in which the recoded ARG8 replaces the COX3 coding sequence complements a deletion of the nuclear ARG8 gene, and its expression requires COX3 mRNA-specific translational activators (8).

Last updated: 1999-10-19

References cited on this page View Complete Literature Guide for ARG8

1)	Heimberg H, et al. (1990) Escherichia coli and Saccharomyces cerevisiae acetylornithine aminotransferase: evolutionary relationship with ornithine aminotransferase. Gene 90(1):69-78
2)	Jauniaux JC, et al. (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
3)	Janssen A and Chen XJ (1998) Cloning, sequencing and disruption of the ARG8 gene encoding acetylornithine aminotransferase in the petite-negative yeast Kluyveromyces lactis. Yeast 14(3):281-5
4)	Messenguy F (1987) Multiplicity of regulatory mechanisms controlling amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Sci 4(5):150-3
5)	Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6)	Messenguy F and Dubois E (1993) Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Cell Biol 13(4):2586-92
7)	Crabeel M, et al. (1995) Further definition of the sequence and position requirements of the arginine control element that mediates repression and induction by arginine in Saccharomyces cerevisiae. Yeast 11(14):1367-80
8)	Steele DF, et al. (1996) Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation. Proc Natl Acad Sci U S A 93(11):5253-7