HRT1/YOL133W Summary Help

Standard Name HRT1 1
Systematic Name YOL133W
Alias RBX1 , ROC1 2 , HRT2
Feature Type ORF, Verified
Description RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair (1, 2, 3, 4, 5, 6, 7, 8)
Name Description High level expression Reduces Ty3 transposition 1
Chromosomal Location
ChrXV:70325 to 70690 | ORF Map | GBrowse
Gene Ontology Annotations All HRT1 GO evidence and references
  View Computational GO annotations for HRT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
reduction of function
134 total interaction(s) for 85 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 39
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 20
  • Co-crystal Structure: 1
  • Co-purification: 3
  • FRET: 1
  • Reconstituted Complex: 29
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 4
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 10

Expression Summary
Length (a.a.) 121
Molecular Weight (Da) 13,940
Isoelectric Point (pI) 5.64
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:70325 to 70690 | ORF Map | GBrowse
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..366 70325..70690 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005493
References cited on this page View Complete Literature Guide for HRT1
1) Seol JH, et al.  (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13(12):1614-26
2) Ohta T, et al.  (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol Cell 3(4):535-41
3) Blondel M, et al.  (2000) Isolation and characterization of HRT1 using a genetic screen for mutants unable to degrade Gic2p in saccharomyces cerevisiae. Genetics 155(3):1033-44
4) Kamura T, et al.  (1999) Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Science 284(5414):657-61
5) Skowyra D, et al.  (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284(5414):662-5
6) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
7) Michel JJ, et al.  (2003) A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem 278(25):22828-37
8) Finley D, et al.  (2012) The Ubiquitin-Proteasome System of Saccharomyces cerevisiae. Genetics 192(2):319-60