Standard Name	HRT1 1 (see Nomenclature conflict Note)
Systematic Name	YOL133W
Alias	RBX1 , ROC1 2 , HRT2
Feature Type	ORF, Verified
Description	RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair (1, 2, 3, 4, 5, 6, 7, 8)
Name Description	High level expression Reduces Ty3 transposition 1

Chromosomal Location	ChrXV:70325 to 70690 | ORF Map | GBrowse

	View Computational GO annotations for HRT1
Molecular Function
Manually curated	protein binding, bridging (IDA) contributes_to ubiquitin-protein ligase activity (IDA, IPI)
Biological Process
Manually curated	G1/S transition of mitotic cell cycle (IMP) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IDA, IMP) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
Cellular Component
Manually curated	Cul3-RING ubiquitin ligase complex (IDA) Cul8-RING ubiquitin ligase complex (IDA) cytoplasm (IDA) nucleus (IDA) SCF ubiquitin ligase complex (IDA, IPI)

Regulatory modules	predicted: stressResponse (421)

Classical genetics
null	inviable
Large-scale survey
null	inviable resistance to sulfanilamide: decreased
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased telomere length: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	127 total interaction(s) for 82 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-RNA: 4 Affinity Capture-Western: 21 Co-crystal Structure: 1 Co-purification: 1 FRET: 1 Reconstituted Complex: 27 Two-hybrid: 14
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 4 Dosage Rescue: 1 Negative Genetic: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 10
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:70325 to 70690 | ORF Map | GBrowse
Last Update	Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..366 70325..70690 2006-01-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005493