MSN1/YOL116W Summary

MSN1/YOL116W Summary

Standard Name	MSN1
Systematic Name	YOL116W
Alias	FUP1 1 , HRB382 , MSS10 2 , 3 , PHD2 4
Feature Type	ORF, Verified
Description	Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress (1, 3, 5, 6, 7, 8)
Name Description	Multicopy suppressor of SNF1 mutation 6

Chromosomal Location	ChrXV:99809 to 100957 \| ORF Map \| GBrowse

Gene Ontology Annotations All MSN1 GO evidence and references

	View Computational GO annotations for MSN1
Molecular Function
Manually curated	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter in response to heat stress (IMP) positive regulation of transcription from RNA polymerase II promoter in response to increased salt (IGI, IMP) positive regulation of transcription from RNA polymerase II promoter in response to methylglyoxal (IMP)
Cellular Component
Manually curated	nucleus (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Regulatory Role


Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All MSN1 Phenotype evidence and references

Classical genetics
null	filamentous growth: decreased pseudohyphal growth: increased resistance to methyl methanesulfonate: decreased
overexpression	pseudohyphal growth: increased
unspecified	resistance to hygromycin B: decreased resistance to papulacandin B: decreased resistance to caffeine: increased
Large-scale survey
null	auxotrophy competitive fitness: increased dessication resistance: decreased filamentous growth: decreased invasive growth: absent lipid particle morphology: abnormal resistance to benzo[a]pyrene: increased resistance to benzo[a]pyrene: decreased starvation resistance: decreased stress resistance: decreased viable
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MSN1 Interaction evidence and references

	90 total interaction(s) for 84 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Two-hybrid: 3
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 12 Negative Genetic: 60 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 6 Synthetic Growth Defect: 2 Synthetic Haploinsufficiency: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MSN1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:99809 to 100957 | |

SGD ORF map

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1149	99809..100957	2006-01-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005476

References cited on this page View Complete Literature Guide for MSN1

1)	Eide D and Guarente L (1992) Increased dosage of a transcriptional activator gene enhances iron-limited growth of Saccharomyces cerevisiae. J Gen Microbiol 138(2):347-54
2)	Lambrechts MG, et al. (1996) A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 repressor gene. Curr Genet 29(6):523-9
3)	Lambrechts MG, et al. (1996) Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast. Proc Natl Acad Sci U S A 93(16):8419-24
4)	Gimeno CJ and Fink GR (1994) Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol Cell Biol 14(3):2100-12
5)	Rep M, et al. (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol 19(8):5474-85
6)	Estruch F and Carlson M (1990) Increased dosage of the MSN1 gene restores invertase expression in yeast mutants defective in the SNF1 protein kinase. Nucleic Acids Res 18(23):6959-64
7)	Chang KS, et al. (2003) The putative transcriptional activator MSN1 promotes chromium accumulation in Saccharomyces cerevisiae. Mol Cells 16(3):291-6
8)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76