MSB4/YOL112W Summary Help

Standard Name MSB4 1
Systematic Name YOL112W
Feature Type ORF, Verified
Description GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Multicopy Suppression of a Budding defect 1, 7
Chromosomal Location
ChrXV:106710 to 108188 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MSB4 GO evidence and references
  View Computational GO annotations for MSB4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
unspecified
Resources
99 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 4
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 9
  • Negative Genetic: 57
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 492
Molecular Weight (Da) 57,092
Isoelectric Point (pI) 5.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:106710 to 108188 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1479 106710..108188 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005472
References cited on this page View Complete Literature Guide for MSB4
1) Iwanejko L, et al.  (1999) Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast 15(14):1529-39
2) Bi E, et al.  (2000) Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell 11(2):773-93
3) Albert S and Gallwitz D  (2000) Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem 381(5-6):453-6
4) Gao XD, et al.  (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol 162(4):635-46
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Bizimungu C and Vandenbol M  (2005) At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity. Biochem Biophys Res Commun 335(3):883-90
7) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80