MSB4/YOL112W Summary Help

MSB4 BASIC INFORMATION

Standard Name MSB4 1
Systematic Name YOL112W
Feature Type ORF, Verified
Description GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization (2, 3, 4)
Name Description Multicopy Suppression of a Budding defect 1, 5
GO Annotations All MSB4 GO evidence and references
    View Computational GO annotations for MSB4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All MSB4 Phenotype details and references
Classical genetics
overexpression
Large-scale survey
null
overexpression
Interactions MSB4 All interactions details and references
31 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 1
Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 5
Sequence Information
ChrXV:106710 to 108188 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1479 106710..108188 2006-01-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005472

MSB4 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
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  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MSB4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MSB4]

1) Iwanejko L, et al.  (1999) Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast 15(14):1529-39
2) Bi E, et al.  (2000) Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell 11(2):773-93
3) Albert S and Gallwitz D  (2000) Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem 381(5-6):453-6
4) Gao XD, et al.  (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol 162(4):635-46
5) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80