MSB4/YOL112W Summary Help

Standard Name MSB4 1
Systematic Name YOL112W
Feature Type ORF, Verified
Description GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Multicopy Suppression of a Budding defect 1, 7
Chromosomal Location
ChrXV:106710 to 108188 | ORF Map | GBrowse
Gene Ontology Annotations All MSB4 GO evidence and references
  View Computational GO annotations for MSB4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 91 genes
Classical genetics
Large-scale survey
99 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 4
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 9
  • Negative Genetic: 57
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 7

Expression Summary
Length (a.a.) 492
Molecular Weight (Da) 57,092
Isoelectric Point (pI) 5.27
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:106710 to 108188 | ORF Map | GBrowse
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1479 106710..108188 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005472
References cited on this page View Complete Literature Guide for MSB4
1) Iwanejko L, et al.  (1999) Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast 15(14):1529-39
2) Bi E, et al.  (2000) Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell 11(2):773-93
3) Albert S and Gallwitz D  (2000) Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem 381(5-6):453-6
4) Gao XD, et al.  (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol 162(4):635-46
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Bizimungu C and Vandenbol M  (2005) At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity. Biochem Biophys Res Commun 335(3):883-90
7) Bender A and Pringle JR  (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A 86(24):9976-80