INO4/YOL108C Summary

INO4 BASIC INFORMATION

Standard Name	INO4
Systematic Name	YOL108C
Feature Type	ORF, Verified
Description	Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain (1, 2)
Name Description	INOsitol requiring

GO Annotations	All INO4 GO evidence and references
	View Computational GO annotations for INO4
Molecular Function
Manually curated	DNA binding (IDA) specific RNA polymerase II transcription factor activity (IMP, IPI, ISS)
Biological Process
Manually curated	phospholipid biosynthetic process (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP, IPI, ISS)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role
Binding motifs	INO4 Transcription Factor Binding Sites and References
	CATGTGAA CATGTGAAAT

Mutant Phenotype	All INO4 Phenotype details and references
Classical genetics
null	GFP-Ino2 distribution: abnormal
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased toxin resistance: decreased viable

Interactions	INO4 All interactions details and references
	107 total interaction(s) for 91 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Far Western: 1 Reconstituted Complex: 2 Two-hybrid: 17
Genetic Interactions	Phenotypic Enhancement: 33 Phenotypic Suppression: 27 Synthetic Growth Defect: 2 Synthetic Lethality: 3 Synthetic Rescue: 2

Sequence Information

ChrXV:111886 to 111431 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..456	111886..111431	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005468

INO4 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for INO4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for INO4]

1)	Schwank S, et al. (1995) Yeast transcriptional activator INO2 interacts as an Ino2p/Ino4p basic helix-loop-helix heteromeric complex with the inositol/choline-responsive element necessary for expression of phospholipid biosynthetic genes in Saccharomyces cerevisiae. Nucleic Acids Res 23(2):230-7
2)	Santiago TC and Mamoun CB (2003) Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p. J Biol Chem 278(40):38723-30

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