PKH2/YOL100W Summary Help

Standard Name PKH2 1
Systematic Name YOL100W
Feature Type ORF, Verified
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Pkb-activating Kinase Homolog 1
Chromosomal Location
ChrXV:129237 to 132482 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PKH2 GO evidence and references
  View Computational GO annotations for PKH2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
103 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 8
  • PCA: 6
  • Reconstituted Complex: 37
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 17
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,081
Molecular Weight (Da) 121,659
Isoelectric Point (pI) 10.46
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:129237 to 132482 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3246 129237..132482 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005460
References cited on this page View Complete Literature Guide for PKH2
1) Casamayor A, et al.  (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
2) Inagaki M, et al.  (1999) PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeast. Mol Cell Biol 19(12):8344-52
3) Friant S, et al.  (2001) Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J 20(23):6783-92
4) Roelants FM, et al.  (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61