SUMMARY PARAGRAPH for AVO1
AVO1 encodes an essential protein that helps maintain the structural integrity of the TOR complex 2 (TORC2) (3). TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (4, 5). In addition to Avo1p, TORC2 contains Avo2p, Tsc11p, Lst8p, Bit61p, Slm1p, Slm2p, and Tor2p (1, 6). Experimental evidence suggests that TORC2 autophosphorylates sites on Avo1p but that this kinase function is not essential to TORC2 integrity (3).
Although the predicted molecular weight of Avo1p is 131 kDa, its apparent molecular weight is 165 kDa (1). Avo1p contains a Ras binding domain and its human homolog hSIN1 (mAvo1p) was originally isolated in a screen for human cDNAs that interfere with S. cerevisiae Ras signaling (7). Not only is Avo1p conserved from yeast to man, but TORC2 is also structurally conserved in higher eukaryotes and, like yeast TORC2, functions to regulate the actin cytoskeleton (1, 8).
Last updated: 2005-11-08