AVO1/YOL078W Summary Help

Standard Name AVO1
Systematic Name YOL078W
Feature Type ORF, Verified
Description Component of a membrane-bound complex containing the Tor2p kinase; contains Tor2p kinase and other proteins; may have a role in regulation of cell growth (1, 2 and see Summary Paragraph)
Name Description Adheres VOraciously (to TOR2)
Chromosomal Location
ChrXV:181682 to 185212 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All AVO1 GO evidence and references
  View Computational GO annotations for AVO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
overexpression
reduction of function
repressible
unspecified
Resources
49 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 11
  • Biochemical Activity: 3
  • Co-purification: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 5
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,176
Molecular Weight (Da) 131,378
Isoelectric Point (pI) 6.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:181682 to 185212 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3531 181682..185212 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005438
SUMMARY PARAGRAPH for AVO1

AVO1 encodes an essential protein that helps maintain the structural integrity of the TOR complex 2 (TORC2) (3). TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (4, 5). In addition to Avo1p, TORC2 contains Avo2p, Tsc11p, Lst8p, Bit61p, Slm1p, Slm2p, and Tor2p (1, 6). Experimental evidence suggests that TORC2 autophosphorylates sites on Avo1p but that this kinase function is not essential to TORC2 integrity (3).

Although the predicted molecular weight of Avo1p is 131 kDa, its apparent molecular weight is 165 kDa (1). Avo1p contains a Ras binding domain and its human homolog hSIN1 (mAvo1p) was originally isolated in a screen for human cDNAs that interfere with S. cerevisiae Ras signaling (7). Not only is Avo1p conserved from yeast to man, but TORC2 is also structurally conserved in higher eukaryotes and, like yeast TORC2, functions to regulate the actin cytoskeleton (1, 8).

Last updated: 2005-11-08 Contact SGD

References cited on this page View Complete Literature Guide for AVO1
1) Loewith R, et al.  (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68
2) Wedaman KP, et al.  (2003) Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(3):1204-20
3) Wullschleger S, et al.  (2005) Molecular organization of target of rapamycin complex 2. J Biol Chem 280(35):30697-704
4) Schmidt A, et al.  (1996) TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A 93(24):13780-5
5) deHart AK, et al.  (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84
6) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
7) Colicelli J, et al.  (1991) Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(7):2913-7
8) Jacinto E, et al.  (2004) Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6(11):1122-8