BRX1/YOL077C Summary Help

Standard Name BRX1 1
Systematic Name YOL077C
Feature Type ORF, Verified
Description Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif (1, 2, 3, 4)
Name Description Xenopus laevis Brix (Biogenesis of Ribosomes in Xenopus) homolog 1
Chromosomal Location
ChrXV:186723 to 185848 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BRX1 GO evidence and references
  View Computational GO annotations for BRX1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
conditional
null
reduction of function
Resources
216 total interaction(s) for 117 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 158
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • PCA: 12
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 6
  • Positive Genetic: 13
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 291
Molecular Weight (Da) 33,531
Isoelectric Point (pI) 9.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:186723 to 185848 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..876 186723..185848 2006-01-05 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005437
References cited on this page View Complete Literature Guide for BRX1
1) Kaser A, et al.  (2001) Brix from xenopus laevis and brx1p from yeast define a new family of proteins involved in the biogenesis of large ribosomal subunits. Biol Chem 382(12):1637-47
2) Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39
3) Bogengruber E, et al.  (2003) Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res 3(1):35-43
4) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27