NUF2/YOL069W Summary Help

Standard Name NUF2 1
Systematic Name YOL069W
Feature Type ORF, Verified
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p (1, 2, 3, 4)
Name Description NUclear Filament-containing protein 1
Chromosomal Location
ChrXV:198942 to 200297 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUF2 GO evidence and references
  View Computational GO annotations for NUF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 67 genes
Resources
Classical genetics
conditional
repressible
Large-scale survey
conditional
null
reduction of function
repressible
unspecified
Resources
226 total interaction(s) for 123 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-Western: 19
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 3
  • PCA: 18
  • Reconstituted Complex: 3
  • Two-hybrid: 147

Genetic Interactions
  • Dosage Rescue: 2
  • Synthetic Lethality: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 451
Molecular Weight (Da) 52,973
Isoelectric Point (pI) 4.35
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:198942 to 200297 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1356 198942..200297 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005430
References cited on this page View Complete Literature Guide for NUF2
1) Osborne MA, et al.  (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66
2) Wigge PA and Kilmartin JV  (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
3) Janke C, et al.  (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91
4) He X, et al.  (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206