Standard Name	RIB2 1
Systematic Name	YOL066C
Alias	PUS8 2
Feature Type	ORF, Verified
Description	Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	RIBoflavin biosynthesis 1

Chromosomal Location	ChrXV:204471 to 202696 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for RIB2
Molecular Function
Manually curated	2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity (IMP) pseudouridine synthase activity (IDA, IMP, ISS)
Biological Process
Manually curated	riboflavin biosynthetic process (IMP) tRNA pseudouridine synthesis (IDA, IMP)
Cellular Component
Manually curated	cytoplasm (ISM)
High-throughput	cytoplasm (IDA)

Pathways	riboflavin, FMN and FAD biosynthesis

Classical genetics
unspecified	auxotrophy 2,5-amino-6, 7-dimethyl-4-oxo-8-ribityl-4,8-dihydropteridine accumulation: increased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
reduction of function	competitive fitness: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	31 total interaction(s) for 30 unique genes/features.
Physical Interactions	Biochemical Activity: 1 Reconstituted Complex: 5 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 15 Phenotypic Enhancement: 1 Positive Genetic: 6 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:204471 to 202696 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1776 204471..202696 2006-01-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005427