GSH2/YOL049W Summary Help

Standard Name GSH2 1, 2
Systematic Name YOL049W
Feature Type ORF, Verified
Description Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock (1, 2, 3)
Name Description Glutathione 1, 2, 4
Chromosomal Location
ChrXV:238619 to 240094 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GSH2 GO evidence and references
  View Computational GO annotations for GSH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
50 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 26
  • Phenotypic Enhancement: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 491
Molecular Weight (Da) 55,815
Isoelectric Point (pI) 5.41
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:238619 to 240094 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1476 238619..240094 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005409
References cited on this page View Complete Literature Guide for GSH2
1) Grant CM, et al.  (1997) Glutathione synthetase is dispensable for growth under both normal and oxidative stress conditions in the yeast Saccharomyces cerevisiae due to an accumulation of the dipeptide gamma-glutamylcysteine. Mol Biol Cell 8(9):1699-707
2) Inoue Y, et al.  (1998) Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae. Biochim Biophys Acta 1395(3):315-20
3) Sugiyama K, et al.  (2000) The Yap1p-dependent induction of glutathione synthesis in heat shock response of Saccharomyces cerevisiae. J Biol Chem 275(20):15535-40
4) OhtakeY, et al.  (1990) Isolation and characterization of glutathione biosynthesis-deficient mutants in Saccharomyces cerevisiae. Agric Biol Chem 54:3145-3150