PEX15/YOL044W Summary Help

Standard Name PEX15 1
Systematic Name YOL044W
Alias PAS21
Feature Type ORF, Verified
Description Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly (1 and see Summary Paragraph)
Name Description PEroXisome related
Chromosomal Location
ChrXV:247150 to 248301 | ORF Map | GBrowse
Gene Ontology Annotations All PEX15 GO evidence and references
  View Computational GO annotations for PEX15
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
reduction of function
Large-scale survey
74 total interaction(s) for 62 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-fractionation: 11
  • PCA: 10
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 31
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 383
Molecular Weight (Da) 43,676
Isoelectric Point (pI) 8.42
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:247150 to 248301 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1152 247150..248301 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005404

Pex15p is a phosphorylated, tail-anchored type II (Ncyt-Clumen) integral peroxisomal membrane protein required for peroxisome biogenesis (1). Pex15p is the first peroxin shown to be posttranslationally modified, but the importance of this phosphorylation for Pex15p function in peroxisome biogenesis is not yet understood (2). Cells lacking Pex15p are characterized by the mislocalization of peroxisomal matrix proteins to the cytosol, while peroxisomal membrane proteins are still targeted to peroxisomal remnants. Overexpression of Pex15p results in impaired peroxisome assembly, and causes a profound proliferation of endomembranes which contain Pex15p as well as at least one other peroxisomal membrane marker (1).

From the continuity of the proliferated membranes with the nuclear envelope, it has been suggested that they most likely originate from the endoplasmic reticulum (ER) (1), but definitive proof of the ER origin of these membranes has not yet been obtained (2). In this respect, the observed O-glycoslylation of overexpressed Pex15p indicates that its carboxy-terminal tail protrudes into ER membranes (1). Further, a Pex15p-invertase fusion protein was shown to be N-glycosylated, even when expressed at the endogenous protein level. From these results, it appears that the association of Pex15p with ER membranes is more likely to reflect a normal step in its topogenesis than an artificial mislocalization caused by its overexpression (2). Thus, Pex15p may be targeted to peroxisomes via the ER, or to both peroxisomes and the ER (1).

Last updated: 2005-03-08 Contact SGD

References cited on this page View Complete Literature Guide for PEX15
1) Elgersma Y, et al.  (1997) Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane. EMBO J 16(24):7326-41
2) Kunau WH and Erdmann R  (1998) Peroxisome biogenesis: back to the endoplasmic reticulum? Curr Biol 8(9):R299-302