NOP12/YOL041C Summary

NOP12 BASIC INFORMATION

Standard Name	NOP12 1
Systematic Name	YOL041C
Feature Type	ORF, Verified
Description	Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p (1, 2, 3)
Name Description	NucleOlar Protein 1

GO Annotations	All NOP12 GO evidence and references
	View Computational GO annotations for NOP12
Molecular Function
Manually curated	RNA binding (ISS)
Biological Process
Manually curated	maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP)
Cellular Component
Manually curated	nucleolus (IDA) preribosome (IDA)

Mutant Phenotype	All NOP12 Phenotype details and references
Classical genetics
null	35S pre-rRNA accumulation: increased 27S rRNA processing intermediate accumulation: increased mature 25S rRNA accumulation: decreased cold sensitivity: increased 60S ribosomal subunit accumulation: decreased viable
Large-scale survey
null	competitive fitness: decreased fermentative growth: decreased rate resistance to 5-fluorouracil: decreased toxin resistance: increased viable

Interactions	NOP12 All interactions details and references
	150 total interaction(s) for 124 unique genes/features.
Physical Interactions	Affinity Capture-MS: 80 Biochemical Activity: 1 Two-hybrid: 2
Genetic Interactions	Phenotypic Enhancement: 15 Phenotypic Suppression: 46 Synthetic Growth Defect: 4 Synthetic Lethality: 2

Sequence Information

ChrXV:252645 to 251266 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1380	252645..251266	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005401

NOP12 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for NOP12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NOP12]

1)	Wu K, et al. (2001) Nucleolar protein Nop12p participates in synthesis of 25S rRNA in Saccharomyces cerevisiae. Nucleic Acids Res 29(14):2938-49
2)	Steffen KK, et al. (2008) Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4. Cell 133(2):292-302
3)	Shirai C and Mizuta K (2008) SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1p. Biosci Biotechnol Biochem 72(7):1881-6

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