TLG2/YOL018C Summary

TLG2 BASIC INFORMATION

Standard Name	TLG2
Systematic Name	YOL018C
Feature Type	ORF, Verified
Description	Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation (1, 2, 3)

GO Annotations	All TLG2 GO evidence and references
	View Computational GO annotations for TLG2
Molecular Function
Manually curated	SNAP receptor activity (TAS)
Biological Process
Manually curated	Golgi to vacuole transport (IGI) inositol phosphorylceramide metabolic process (IGI, IMP) mannose inositol phosphoceramide metabolic process (IGI, IMP) protein exit from endoplasmic reticulum (IGI) vesicle fusion (TAS)
Cellular Component
Manually curated	early endosome (TAS) Golgi trans cisterna (TAS) trans-Golgi network (TAS)

Mutant Phenotype	All TLG2 Phenotype details and references
Classical genetics
null	ceramide phosphoinositols accumulation: decreased killer toxin resistance: decreased metal resistance: decreased carboxypeptidase Y (Prc1p) modification: decreased resistance to monensin: decreased resistance to brefeldin A: decreased resistance to gentamycins: decreased resistance to Papulacandin B: decreased resistance to Echinocandin B: decreased resistance to calcium dichloride: decreased resistance to caffeine: decreased toxin resistance: decreased
Large-scale survey
null	competitive fitness: decreased metal resistance: decreased resistance to rapamycin: decreased resistance to wortmannin: normal resistance to hygromycin B: decreased resistance to ethylenediaminetetraacetic acid: decreased resistance to 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to caffeine: decreased resistance to nordihydroguaiaretic acid: decreased resistance to chlorpromazine: decreased resistance to cesium chloride: decreased resistance to cycloheximide: decreased resistance to diltiazem-HCl: decreased resistance to latrunculin B: decreased resistance to ruthenium red: decreased resistance to rubidium chloride: decreased resistance to strontium dichloride: decreased resistance to lithium chloride: decreased resistance to monensin: decreased resistance to nickel sulfate: increased toxin resistance: decreased viable
overexpression	resistance to rapamycin: decreased

Interactions	TLG2 All interactions details and references
	249 total interaction(s) for 142 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 21 Biochemical Activity: 2 Co-purification: 35 Reconstituted Complex: 4 Two-hybrid: 11
Genetic Interactions	Phenotypic Enhancement: 120 Phenotypic Suppression: 16 Synthetic Growth Defect: 24 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 11

Sequence Information

ChrXV:292075 to 290882 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1194	292075..290882	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005378

TLG2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for TLG2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for TLG2]

1)	Coe JG, et al. (1999) A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae. Mol Biol Cell 10(7):2407-23
2)	Bryant NJ and James DE (2001) Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation. EMBO J 20(13):3380-8
3)	Dulubova I, et al. (2002) How Tlg2p/syntaxin 16 'snares' Vps45. EMBO J 21(14):3620-31

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