LYS9/YNR050C Summary Help

Standard Name LYS9 1
Systematic Name YNR050C
Alias LYS13
Feature Type ORF, Verified
Description Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 (2, 3 and see Summary Paragraph)
Name Description LYSine requiring 1
Chromosomal Location
ChrXIV:715388 to 714048 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 44 cM
Gene Ontology Annotations All LYS9 GO evidence and references
  View Computational GO annotations for LYS9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 12 genes
Classical genetics
Large-scale survey
20 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Two-hybrid: 4

Genetic Interactions
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 446
Molecular Weight (Da) 48,917
Isoelectric Point (pI) 4.95
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:715388 to 714048 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 44 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1341 715388..714048 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005333

About lysine biosynthesis

S. cerevisiae synthesizes the essential amino acid L-lysine via the L-alpha-aminoadipic acid pathway instead of the diaminopmelate pathway (4). Originally proposed to be characteristic of fungi, recent studies suggest prokaryotes also synthesize lysine via the alpha-aminoadipic acid pathway (5). Intermediates in this pathway are often incorporated into secondary metabolites. For example, it has been well- studied that alpha-aminoadipate is required for penicillin production (4). Regulation of the lysine biosynthetic pathway in S. cerevisiae is an interaction between general amino acid control (via Gcn4p) (6), feedback inhibition of homocitrate synthase activity by lysine (7), and induction of Lys14p by alpha-aminoadipate semialdehyde (8).

Last updated: 2007-10-04 Contact SGD

References cited on this page View Complete Literature Guide for LYS9
1) Borell CW, et al.  (1984) Two unlinked lysine genes (LYS9 and LYS14) are required for the synthesis of saccharopine reductase in Saccharomyces cerevisiae. J Bacteriol 159(1):429-32
2) Storts DR and Bhattacharjee JK  (1987) Purification and properties of saccharopine dehydrogenase (glutamate forming) in the Saccharomyces cerevisiae lysine biosynthetic pathway. J Bacteriol 169(1):416-8
3) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77
4) Zabriskie TM and Jackson MD  (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17(1):85-97
5) Nishida H and Nishiyama M  (2000) What is characteristic of fungal lysine synthesis through the alpha-aminoadipate pathway? J Mol Evol 51(3):299-302
6) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Feller A, et al.  (1999) In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur J Biochem 261(1):163-70
8) El Alami M, et al.  (2000) Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae. Curr Genet 38(2):78-86