Standard Name	TRM112 1
Systematic Name	YNR046W
Feature Type	ORF, Verified
Description	Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4, 5)
Name Description	TRna Methyltransferase 1

Chromosomal Location	ChrXIV:707788 to 708195 | ORF Map | GBrowse

	View Computational GO annotations for TRM112
Molecular Function
Manually curated	contributes_to protein methyltransferase activity (IDA) contributes_to rRNA (guanine) methyltransferase activity (IMP, IPI) contributes_to tRNA (guanine-N2-)-methyltransferase activity (IDA, IMP) zinc ion binding (ISS)
Biological Process
Manually curated	peptidyl-glutamine methylation (IDA) rRNA (guanine-N7)-methylation (IMP) tRNA methylation (IMP)
Cellular Component
Manually curated	eRF1 methyltransferase complex (IPI) tRNA (m2G10) methyltransferase complex (IPI)
High-throughput	cytoplasm (IDA) nucleolus (IDA) nucleus (IDA)

Classical genetics
null	35S pre-rRNA accumulation: increased 18S rRNA modification: absent cold sensitivity: increased growth in exponential phase: decreased rate ribosomal subunit accumulation: abnormal resistance to paromomycin: decreased toxin resistance: increased
repressible	toxin resistance: increased
Large-scale survey
null	haploproficient inviable vegetative growth: decreased
reduction of function	competitive fitness: decreased
repressible	cell cycle progression in G1 phase: abnormal mitochondrial morphology: abnormal
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	95 total interaction(s) for 70 unique genes/features.
Physical Interactions	Affinity Capture-MS: 59 Affinity Capture-RNA: 2 Affinity Capture-Western: 10 Co-purification: 1 PCA: 2 Protein-RNA: 1 Reconstituted Complex: 8 Two-hybrid: 4
Genetic Interactions	Negative Genetic: 2 Synthetic Growth Defect: 2 Synthetic Lethality: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:707788 to 708195 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..408 707788..708195 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005329