MVD1/YNR043W Summary

Standard Name	MVD1 1
Systematic Name	YNR043W
Alias	ERG19 2
Feature Type	ORF, Verified
Description	Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer (2, 3, 4 and see Summary Paragraph)
Name Description	MeValonate pyrophosphate Decarboxylase

Chromosomal Location	ChrXIV:701895 to 703085 \| ORF Map \| GBrowse

Gene Ontology Annotations All MVD1 GO evidence and references

	View Computational GO annotations for MVD1
Molecular Function
Manually curated	diphosphomevalonate decarboxylase activity (IDA, IMP)
Biological Process
Manually curated	sterol biosynthetic process (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Pathways	mevalonate pathway superpathway of ergosterol biosynthesis

Mutant phenotypes All MVD1 Phenotype evidence and references

Classical genetics
conditional	heat sensitivity: increased resistance to stichloroside: increased resistance to theopalauamide: increased
Large-scale survey
conditional	heat sensitivity: increased resistance to hydroxyurea: decreased
null	haploproficient inviable resistance to sulfanilamide: decreased starvation resistance: decreased
reduction of function	competitive fitness: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MVD1 Interaction evidence and references

	31 total interaction(s) for 29 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10
Genetic Interactions	Negative Genetic: 14 Positive Genetic: 5 Synthetic Growth Defect: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MVD1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:701895 to 703085 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1191	701895..703085	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005326

SUMMARY PARAGRAPH for MVD1

MVD1 encodes diphosphomevalonate decarboxylase, which converts mevalonate-5-pyrophosphate to isopentenyl pyrophosphate in the biosynthesis of isoprenoids and sterols, including ergosterol in yeast (2, 5, 6). MVD1 is essential for viability; a temperature-sensitive mvd1 mutation is auxotrophic for ergosterol and lacks diphosphomevalonate decarboxylase activity (2, 4). Mvd1p acts as a homodimer; the temperature-sensitive erg19-34 allele impairs dimerization (3).

Mvd1p is a member of the GHMP kinase superfamily, which includes small-molecule kinases such as galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (7, 8). The human diphosphomevalonate decarboxylase has been cloned and sequenced, and is a possible target in the treatment of hypercholesterolemia (5). The Arabidopsis enzyme, encoded by AtMVD1, complements S. cerevisiae mvd1 mutant phenotypes (9).

Last updated: 2009-07-29

References cited on this page View Complete Literature Guide for MVD1

1)	SGD (2009) Gene name standardized from a GenBank/EMBL/DDBJ record
2)	Chambon C, et al. (1991) Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase. Lipids 26(8):633-6
3)	Cordier H, et al. (1999) The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase (erg19p) forms homodimers in vivo, and a single substitution in a structurally conserved region impairs dimerization. Curr Microbiol 38(5):290-4
4)	Berges T, et al. (1997) The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity. J Bacteriol 179(15):4664-70
5)	Toth MJ and Huwyler L (1996) Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase. J Biol Chem 271(14):7895-8
6)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
7)	Bonanno JB, et al. (2001) Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis. Proc Natl Acad Sci U S A 98(23):12896-901
8)	Krepkiy DV and Miziorko HM (2005) Investigation of the functional contributions of invariant serine residues in yeast mevalonate diphosphate decarboxylase. Biochemistry 44(7):2671-7
9)	Cordier H, et al. (1999) Heterologous expression in Saccharomyces cerevisiae of an Arabidopsis thaliana cDNA encoding mevalonate diphosphate decarboxylase. Plant Mol Biol 39(5):953-67