ABZ1/YNR033W Summary Help

Standard Name ABZ1
Systematic Name YNR033W
Feature Type ORF, Verified
Description Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description para-AminoBenZoic acid (PABA) biosynthesis
Chromosomal Location
ChrXIV:687635 to 689998 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ABZ1 GO evidence and references
  View Computational GO annotations for ABZ1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Pathways
Large-scale survey
null
Resources
52 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 3
  • Co-purification: 1
  • Protein-peptide: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 33
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 787
Molecular Weight (Da) 88,543
Isoelectric Point (pI) 5.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:687635 to 689998 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2364 687635..689998 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005316
SUMMARY PARAGRAPH for ABZ1

ABZ1 encodes a cytoplasmic protein (5) required for synthesis of para-aminobenzoic acid (PABA), an important component in folic acid synthesis (1, 2). Specifically, Abz1p is aminodeoxychorismate synthase (E.C. 6.3.5.8), which converts chorismate to 4-amino-4-deoxychorismate. ABZ1p resembles a fusion of the E. coli proteins pabA and pabB, which together form a complex that performs the same reaction (1). In E. coli, a third enzyme, pabC (E.C. 4.1.3.38), converts aminodeoxychorismate to PABA. Deletion of ABZ1 causes PABA auxotrophy, but mutants cultured in PABA-supplemented medium grow normally (1).

Sulfa drugs, which are often used to treat AIDS patients with opportunistic microbial infections, compete with PABA in the folic acid synthesis pathway (2). Overexpression of ABZ1 confers sulfa resistance in Saccharomyces cerevisiae (1, 2).

Last updated: 2005-08-25 Contact SGD

References cited on this page View Complete Literature Guide for ABZ1
1) Edman JC, et al.  (1993) Para-aminobenzoate synthase gene of Saccharomyces cerevisiae encodes a bifunctional enzyme. Yeast 9(6):669-75
2) Castelli LA, et al.  (2001) Sulfa drug screening in yeast: fifteen sulfa drugs compete with p-aminobenzoate in Saccharomyces cerevisiae. FEMS Microbiol Lett 199(2):181-4
3) Marbois B, et al.  (2010) para-Aminobenzoic acid is a precursor in coenzyme Q6 biosynthesis in Saccharomyces cerevisiae. J Biol Chem 285(36):27827-38
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91