SSK2/YNR031C Summary Help

Standard Name SSK2 1
Systematic Name YNR031C
Feature Type ORF, Verified
Description MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Suppressor of Sensor Kinase 1
Chromosomal Location
ChrXIV:685433 to 680694 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SSK2 GO evidence and references
  View Computational GO annotations for SSK2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
284 total interaction(s) for 158 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Biochemical Activity: 4
  • PCA: 4
  • Reconstituted Complex: 12
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 12
  • Dosage Lethality: 2
  • Dosage Rescue: 6
  • Negative Genetic: 172
  • Phenotypic Enhancement: 4
  • Positive Genetic: 17
  • Synthetic Growth Defect: 8
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,579
Molecular Weight (Da) 180,526
Isoelectric Point (pI) 5.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:685433 to 680694 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4740 685433..680694 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005314
References cited on this page View Complete Literature Guide for SSK2
1) Maeda T, et al.  (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369(6477):242-5
2) Posas F and Saito H  (1998) Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator. EMBO J 17(5):1385-94
3) Yuzyuk T, et al.  (2002) The MEK kinase Ssk2p promotes actin cytoskeleton recovery after osmotic stress. Mol Biol Cell 13(8):2869-80
4) Tatebayashi K, et al.  (2003) A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway. EMBO J 22(14):3624-34
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Lavina WA, et al.  (2014) Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2?msg5? double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1. J Biosci Bioeng 117(2):135-41