RCF2/YNR018W Summary Help

Standard Name RCF2 1
Systematic Name YNR018W
Alias AIM38 2
Feature Type ORF, Verified
Description Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast (1, 3, 4)
Name Description Respiratory superComplex Factor 1
Chromosomal Location
ChrXIV:664270 to 664944 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RCF2 GO evidence and references
  View Computational GO annotations for RCF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 10 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
55 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 19
  • Co-purification: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 21
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 224
Molecular Weight (Da) 25,344
Isoelectric Point (pI) 10.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:664270 to 664944 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..675 664270..664944 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005301
References cited on this page View Complete Literature Guide for RCF2
1) Strogolova V, et al.  (2012) Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol 32(8):1363-73
2) Hess DC, et al.  (2009) Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis. PLoS Genet 5(3):e1000407
3) Helbig AO, et al.  (2009) A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics 9(20):4787-98
4) Vukotic M, et al.  (2012) Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15(3):336-47