URK1/YNR012W Summary Help

Standard Name URK1 1
Systematic Name YNR012W
Feature Type ORF, Verified
Description Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP (2, 3, 4 and see Summary Paragraph)
Name Description URidine Kinase 1
Chromosomal Location
ChrXIV:647432 to 648937 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All URK1 GO evidence and references
  View Computational GO annotations for URK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
28 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 5
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 501
Molecular Weight (Da) 56,296
Isoelectric Point (pI) 7.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:647432 to 648937 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1506 647432..648937 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005295
SUMMARY PARAGRAPH for URK1

Urk1p is a uridine kinase (EC 2.7.1.48) that phosphorylates uridine into UMP and cytidine into CMP in the pyrimidine ribonucleotide salvage pathway (1, 2). Urk1p can also phosphorylate deoxycytidine into dCMP in pyrimidine deoxyribonucleotide salvage (1, 2). These two pathways provide pyrimidines required for nucleic acid synthesis, amino acid synthesis, and energy (2).

URK1 is repressed in response to lithium chloride during growth on galactose (5). urk1 null mutants grow well on uridine and cytosine, but are unable to utilize cytidine, and are also resistant to 5-fluorocytidine (5-FCR) and 5-fluorouridine (5-FUR) (2, 4). urh1 urk1 double mutants are unable to utilize uridine as the sole source of pyrimidines (3).

Urk1p displays similarity to the rat myocardial uridine kinase (2).

Last updated: 2005-11-29 Contact SGD

References cited on this page View Complete Literature Guide for URK1
1) Kern L  (1990) The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase. Nucleic Acids Res 18(17):5279
2) Kurtz JE, et al.  (1999) New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism. Curr Genet 36(3):130-6
3) Mitterbauer R, et al.  (2002) Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase. Appl Environ Microbiol 68(3):1336-43
4) Kurtz JE, et al.  (2002) The URH1 uridine ribohydrolase of Saccharomyces cerevisiae. Curr Genet 41(3):132-41
5) Mitterbauer R, et al.  (2003) A sensitive and inexpensive yeast bioassay for the mycotoxin zearalenone and other compounds with estrogenic activity. Appl Environ Microbiol 69(2):805-11